Screening for ligninolytic enzyme production by diverse fungi from Tunisia

This work represents the first report on the ability of autochthonous fungi from Tunisia to produce ligninolytic enzymes. Three hundred and fifteen fungal strains were isolated from different Tunisian biotopes. These fungal strains were firstly screened on solid media containing Poly R-478 or ABTS a...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2005-12, Vol.21 (8-9), p.1415-1423
Main Authors: DHOUIB, Abdelhafidh, HAMZA, Manel, ZOUARI, Hela, MECHICHI, Tahar, HMIDI, Rafik, LABAT, Marc, MARTINEZ, Maria Jesus, SAYADI, Sami
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Biotechnology
Biotopes
Enzymes
Fundamental and applied biological sciences. Psychology
Lignin
Manganese
Microbiology
Oxyporus
Ribosomal DNA
Stereum
Trametes
Trichoderma
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Summary:This work represents the first report on the ability of autochthonous fungi from Tunisia to produce ligninolytic enzymes. Three hundred and fifteen fungal strains were isolated from different Tunisian biotopes. These fungal strains were firstly screened on solid media containing Poly R-478 or ABTS as indicator compounds that enabled the detection of lignin-modifying enzymes as specific color reactions. Of the 315 tested strains, 49 exhibited significant ABTS-oxidation activity expressed within the first week of incubation and only 18 strains decolorized the Poly R-478. Liquid cultivations and laccase, manganese peroxidase and lignin peroxidase activity assays of positive strains confirmed that eight efficient enzyme producers were found in the screening. These strains were attributed to the most closely related species using PCR amplification and sequencing of the internal transcribed spacer 'ITS' regions of the ribosomal DNA. The identification results showed fungal genera such as Oxyporus, Stereum and Trichoderma which have been only rarely reported as ligninolytic enzyme producers in the literature. Culture conditions and medium composition were optimized for the laccase producer Trametes trogii CTM 10156. This optimization resulted in high laccase production, 367 times more than in non-optimized conditions and which reached 110 U ml^sup -1^ within 15 days of incubation.[PUBLICATION ABSTRACT]
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-005-5774-z