molecular probe for Basidiomycota: the spermidine synthase-saccharopine dehydrogenase chimeric gene

By means of an in silico analysis, we demonstrated that a previously described chimeric gene (Spe-Sdh) encoding spermidine synthase, a key enzyme involved in the synthesis of polyamines, and saccharopine dehydrogenase, an enzyme involved in lysine synthesis in fungi, were present exclusively in memb...

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Bibliographic Details
Published in:FEMS microbiology letters 2010-11, Vol.312 (1), p.77-83
Main Authors: León-Ramírez, Claudia G, Valdés-Santiago, Laura, Campos-Góngora, Eduardo, Ortiz-Castellanos, Lucila, Aréchiga-Carvajal, Elva T, Ruiz-Herrera, José
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amplification
Base Sequence
Basidiomycota
Basidiomycota - chemistry
Basidiomycota - classification
Basidiomycota - enzymology
Basidiomycota - genetics
Biological and medical sciences
chimeric gene
Dehydrogenase
Dehydrogenases
DNA Primers - genetics
Enzymes
Fundamental and applied biological sciences. Psychology
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - metabolism
Fungi
Lysine
Microbiology
Miscellaneous
molecular probe
Molecular Probes - genetics
Molecular Sequence Data
Mycology
Phylogeny
Polyamines
Polymerase Chain Reaction
Primers
Saccharopine dehydrogenase
Saccharopine Dehydrogenases - chemistry
Saccharopine Dehydrogenases - genetics
Saccharopine Dehydrogenases - metabolism
Sequence Alignment
Species
Species Specificity
Spermidine
Spermidine synthase
Spermidine Synthase - chemistry
Spermidine Synthase - genetics
Spermidine Synthase - metabolism
Synthesis
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Description
Summary:By means of an in silico analysis, we demonstrated that a previously described chimeric gene (Spe-Sdh) encoding spermidine synthase, a key enzyme involved in the synthesis of polyamines, and saccharopine dehydrogenase, an enzyme involved in lysine synthesis in fungi, were present exclusively in members of all Basidiomycota subphyla, but not in any other group of living organisms. We used this feature to design degenerated primers to amplify a specific fragment of the Spe-Sdh gene by PCR, as a tool to unequivocally identify Basidiomycota isolates. The specificity of this procedure was tested using different fungal species. As expected, positive results were obtained only with Basidiomycota species, whereas no amplification was achieved with species belonging to other fungal phyla.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2010.02099.x