Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods

Typing methods to evaluate isolates in relation to their phenotypical and molecular characteristics are essential in epidemiological studies. In this study, Candida albicans biotypes were determined before and after storage in order to verify their stability. Twenty C. albicans isolates were typed b...

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Bibliographic Details
Published in:Mycoses 2010-11, Vol.53 (6), p.468-474
Main Authors: Bacelo, Katia Leston, da Costa, Karen Regina Carim, Ferreira, Joseane Cristina, Candido, Regina Celia
Format: Article
Language:English
Subjects:
Candida
Candida albicans
Candida albicans - classification
Candida albicans - genetics
Candida albicans - metabolism
DNA, Fungal - genetics
Fungal Proteins - metabolism
Genomic Instability
Genotype
Humans
morphotyping
Mycological Typing Techniques
Mycology - methods
Peptide Hydrolases - metabolism
Phenotype
phospholipase
Phospholipases - metabolism
Preservation, Biological
proteinase
Random Amplified Polymorphic DNA Technique
RAPD
Specimen Handling - methods
storage
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Summary:Typing methods to evaluate isolates in relation to their phenotypical and molecular characteristics are essential in epidemiological studies. In this study, Candida albicans biotypes were determined before and after storage in order to verify their stability. Twenty C. albicans isolates were typed by Randomly Amplified Polymorphic DNA (RAPD), production of phospholipase and proteinase exoenzymes (enzymotyping) and morphotyping before and after 180 days of storage in Sabouraud dextrose agar (SDA) and sterilised distilled water. Before the storage, 19 RAPD patterns, two enzymotypes and eight morphotypes were identified. The fragment patterns obtained by RAPD, on the one hand, were not significantly altered after storage. On the other hand, the majority of the isolates changed their enzymotype and morphotype after storage. RAPD typing provided the better discriminatory index (DI) among isolates (DI = 0.995) and maintained the profile identified, thereby confirming its utility in epidemiological surveys. Based on the low reproducibility observed after storage in SDA and distilled water by morphotyping (DI = 0.853) and enzymotyping (DI = 0.521), the use of these techniques is not recommended on stored isolates.
ISSN:0933-7407
1439-0507
DOI:10.1111/j.1439-0507.2009.01741.x