A novel peptide derivative exhibits antiinflammatory and antioxidant activity in adjuvant induced arthritis in rats

• Published in: Molecular and cellular biochemistry 2002-01, Vol.229 (1-2), p.9-17
• Main Authors: Kumar, Dilly Ashok, Manikandan, Panchatcharam, Sumitra, Miriyala, Narayana Raju, Kalidindi Vs, Gayathri, Chandrasekar, Arutselvan, Natarajan, Puvanakrishnan, Rengarajulu
• Format: Article
• Language: English
• Subjects: Antioxidants; Free radicals; Mycobacterium tuberculosis; Rodents
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1023/A:1017980024029

A tetrapeptide derivative PEP1261 {Boc-Lys-(Boc)-Arg-Asp-Ser-(tBu)-OtBu}, corresponding to residues 39-42 of human lactoferrin, was tested for its antiinflammatory action in adjuvant induced arthritis in rats. Administration of heat killed Mycobacterium tuberculosis (500 μg/0.1 ml of paraffin oil) intradermally into the foot pad of right hind paw resulted in an increased paw volume and an increase in the levels of reactive oxygen species and β-glucuronidase as well as a decrease in the antioxidants levels. PEP1261, at an effective dose of 10 mg/kg body wt., exhibited a significant antiarthritic activity as evidenced by lowering of paw volume and inhibited the free radicals toxicity by increasing the antioxidants levels. This peptide derivative was also shown to have a membrane stabilizing action by significantly decreasing the total and free activity of β-glucuronidase and inhibiting the rate of release of the enzyme from lysosomal rich fraction. Histopathological studies confirmed the above results by showing a decrease in mononuclear cell infiltration, hypertrophy, hyperplasia and pannus formation after PEP1261 treatment in arthritic rats.[PUBLICATION ABSTRACT]