Loading…
Nickel deficiency diminishes sperm quantity and movement in rats
Early studies on nickel essentiality with rats and goats indicated that nickel deprivation impaired reproductive performance. Nickel also has been found to influence cyclic nucleotide gated channels (CNG); these types of channels are important in sperm physiology. Thus, two experiments were conducte...
Saved in:
Published in: | Biological trace element research 2003, Vol.93 (1-3), p.141-153 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c442t-c55dcc024150894c929ac43cd09dd3e397326cb65c09cac2b2d64e4ae13c82583 |
---|---|
cites | |
container_end_page | 153 |
container_issue | 1-3 |
container_start_page | 141 |
container_title | Biological trace element research |
container_volume | 93 |
creator | Yokoi, Katsuhiko Uthus, Eric O Nielsen, Forrest H |
description | Early studies on nickel essentiality with rats and goats indicated that nickel deprivation impaired reproductive performance. Nickel also has been found to influence cyclic nucleotide gated channels (CNG); these types of channels are important in sperm physiology. Thus, two experiments were conducted to test the hypothesis that nickel deficiency affects sperm physiology in a manner consistent with nickel having an essential function related to CNG channel functions. The experiments were factorially arranged with four treatment groups of eight weanling rats in each. In experiment 1, the treatments were supplemental dietary nickel of 0 and 1 mg/kg and N(ω)-nitro-l-arginine methyl ester (l-NAME, a nitric oxide synthase inhibitor) added to the drinking water (50 mg/100 mL) the last 3 wk of an 8-wk experiment. In experment 2, the treatments were supplemental dietary nickel at 0 and 1 mg/kg and supplemental dietary sodium chloride (NaCl) at 0 and 80 g/kg. The NaCl and l-NAME variables were included to act as stressors affecting CNG channel activity. The basal diet contained per kilogram about 27 µg of nickel and 1 g of sodium. After 8 wk in experiment 1 and 16 wk in experiment 2, urine while fasting and testes and epididymis in both experiments, and seminal vesicles and prostates in experiment 2 were harvested for analysis. Nickel deprivation significantly decreased spermatozoa motility and density in the epididymides, epididymal transit time of spermatozoa, and testes sperm production rate. Nickel deficiency also significantly decreased the weights of the seminal vesicles and prostate glands. Excessive NaCl had no effect on sperm physiology; however, it decreased prostate gland weights. The findings support the hypothesis that nickel has an essential function that possibly could affect reproductive performance in higher animals, perhaps through affecting a CNG channel function. |
doi_str_mv | 10.1385/bter:93:1-3:141 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_815538255</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1940873821</sourcerecordid><originalsourceid>FETCH-LOGICAL-c442t-c55dcc024150894c929ac43cd09dd3e397326cb65c09cac2b2d64e4ae13c82583</originalsourceid><addsrcrecordid>eNp90UtPGzEQAGCrAjU8eu6NrjjQ0zZ-7to5AVFakFCR2uRsOeMJNWR3g72LlH-Pq0RqxYHDaObwzUgzQ8hnRr8xodV42WOcGDFhZQ7JPpAjppQpac3pwX_1iByn9Egpq7kRH8mIcS2UNPqIXP4M8ITrwuMqQMAWtoUPTWhD-oOpSBuMTfE8uLYP_bZwrS-a7gUbbPsitEV0fTolhyu3Tvhpn0_I4vtsPr0p7-5_3E6v7kqQkvclKOUBKJdMUW0kGG4cSAGeGu8FClMLXsGyUkANOOBL7iuJ0iEToLnS4oR83c3dxO55wNTbJiTA9dq12A3J6rysyFJlefGurIWsDec8w_M38LEbYpu3sJxpUUmpq4zGOwSxSyniym5iaFzcWkbt3x_Y6_nslzXCMptDstxxth87LBv0__z-6Bl82YGV66x7iCHZxW9OmaDMMKqrWrwC-veKig</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>218364486</pqid></control><display><type>article</type><title>Nickel deficiency diminishes sperm quantity and movement in rats</title><source>Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List</source><creator>Yokoi, Katsuhiko ; Uthus, Eric O ; Nielsen, Forrest H</creator><creatorcontrib>Yokoi, Katsuhiko ; Uthus, Eric O ; Nielsen, Forrest H</creatorcontrib><description>Early studies on nickel essentiality with rats and goats indicated that nickel deprivation impaired reproductive performance. Nickel also has been found to influence cyclic nucleotide gated channels (CNG); these types of channels are important in sperm physiology. Thus, two experiments were conducted to test the hypothesis that nickel deficiency affects sperm physiology in a manner consistent with nickel having an essential function related to CNG channel functions. The experiments were factorially arranged with four treatment groups of eight weanling rats in each. In experiment 1, the treatments were supplemental dietary nickel of 0 and 1 mg/kg and N(ω)-nitro-l-arginine methyl ester (l-NAME, a nitric oxide synthase inhibitor) added to the drinking water (50 mg/100 mL) the last 3 wk of an 8-wk experiment. In experment 2, the treatments were supplemental dietary nickel at 0 and 1 mg/kg and supplemental dietary sodium chloride (NaCl) at 0 and 80 g/kg. The NaCl and l-NAME variables were included to act as stressors affecting CNG channel activity. The basal diet contained per kilogram about 27 µg of nickel and 1 g of sodium. After 8 wk in experiment 1 and 16 wk in experiment 2, urine while fasting and testes and epididymis in both experiments, and seminal vesicles and prostates in experiment 2 were harvested for analysis. Nickel deprivation significantly decreased spermatozoa motility and density in the epididymides, epididymal transit time of spermatozoa, and testes sperm production rate. Nickel deficiency also significantly decreased the weights of the seminal vesicles and prostate glands. Excessive NaCl had no effect on sperm physiology; however, it decreased prostate gland weights. The findings support the hypothesis that nickel has an essential function that possibly could affect reproductive performance in higher animals, perhaps through affecting a CNG channel function.</description><identifier>ISSN: 1559-0720</identifier><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>EISSN: 0163-4984</identifier><identifier>DOI: 10.1385/bter:93:1-3:141</identifier><identifier>PMID: 12835498</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>animal reproduction ; Animals ; cyclic nucleotide-gated channels ; deficiency diseases ; Diet ; dietary mineral supplements ; Dietary Supplements ; Drinking water ; enzyme inhibitors ; epididymis ; Epididymis - cytology ; Epididymis - drug effects ; Epididymis - physiology ; Experiments ; Male ; NG-Nitroarginine Methyl Ester - administration & dosage ; NG-Nitroarginine Methyl Ester - pharmacology ; Nickel ; Nickel - administration & dosage ; Nickel - deficiency ; Nickel - pharmacology ; Nitric oxide ; nitric oxide synthase ; nutrient deficiencies ; Organ Size - drug effects ; Physiology ; prostate gland ; Rats ; Rats, Sprague-Dawley ; Reproduction ; Rodents ; Sodium chloride ; Sodium Chloride, Dietary - pharmacology ; Sperm Count ; sperm motility ; Sperm Motility - drug effects ; Sperm Motility - physiology ; spermatozoa ; Spermatozoa - cytology ; Spermatozoa - drug effects ; Spermatozoa - physiology ; testes ; weanlings</subject><ispartof>Biological trace element research, 2003, Vol.93 (1-3), p.141-153</ispartof><rights>Humana Press Inc. 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-c55dcc024150894c929ac43cd09dd3e397326cb65c09cac2b2d64e4ae13c82583</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12835498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yokoi, Katsuhiko</creatorcontrib><creatorcontrib>Uthus, Eric O</creatorcontrib><creatorcontrib>Nielsen, Forrest H</creatorcontrib><title>Nickel deficiency diminishes sperm quantity and movement in rats</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><description>Early studies on nickel essentiality with rats and goats indicated that nickel deprivation impaired reproductive performance. Nickel also has been found to influence cyclic nucleotide gated channels (CNG); these types of channels are important in sperm physiology. Thus, two experiments were conducted to test the hypothesis that nickel deficiency affects sperm physiology in a manner consistent with nickel having an essential function related to CNG channel functions. The experiments were factorially arranged with four treatment groups of eight weanling rats in each. In experiment 1, the treatments were supplemental dietary nickel of 0 and 1 mg/kg and N(ω)-nitro-l-arginine methyl ester (l-NAME, a nitric oxide synthase inhibitor) added to the drinking water (50 mg/100 mL) the last 3 wk of an 8-wk experiment. In experment 2, the treatments were supplemental dietary nickel at 0 and 1 mg/kg and supplemental dietary sodium chloride (NaCl) at 0 and 80 g/kg. The NaCl and l-NAME variables were included to act as stressors affecting CNG channel activity. The basal diet contained per kilogram about 27 µg of nickel and 1 g of sodium. After 8 wk in experiment 1 and 16 wk in experiment 2, urine while fasting and testes and epididymis in both experiments, and seminal vesicles and prostates in experiment 2 were harvested for analysis. Nickel deprivation significantly decreased spermatozoa motility and density in the epididymides, epididymal transit time of spermatozoa, and testes sperm production rate. Nickel deficiency also significantly decreased the weights of the seminal vesicles and prostate glands. Excessive NaCl had no effect on sperm physiology; however, it decreased prostate gland weights. The findings support the hypothesis that nickel has an essential function that possibly could affect reproductive performance in higher animals, perhaps through affecting a CNG channel function.</description><subject>animal reproduction</subject><subject>Animals</subject><subject>cyclic nucleotide-gated channels</subject><subject>deficiency diseases</subject><subject>Diet</subject><subject>dietary mineral supplements</subject><subject>Dietary Supplements</subject><subject>Drinking water</subject><subject>enzyme inhibitors</subject><subject>epididymis</subject><subject>Epididymis - cytology</subject><subject>Epididymis - drug effects</subject><subject>Epididymis - physiology</subject><subject>Experiments</subject><subject>Male</subject><subject>NG-Nitroarginine Methyl Ester - administration & dosage</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nickel</subject><subject>Nickel - administration & dosage</subject><subject>Nickel - deficiency</subject><subject>Nickel - pharmacology</subject><subject>Nitric oxide</subject><subject>nitric oxide synthase</subject><subject>nutrient deficiencies</subject><subject>Organ Size - drug effects</subject><subject>Physiology</subject><subject>prostate gland</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reproduction</subject><subject>Rodents</subject><subject>Sodium chloride</subject><subject>Sodium Chloride, Dietary - pharmacology</subject><subject>Sperm Count</subject><subject>sperm motility</subject><subject>Sperm Motility - drug effects</subject><subject>Sperm Motility - physiology</subject><subject>spermatozoa</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>testes</subject><subject>weanlings</subject><issn>1559-0720</issn><issn>0163-4984</issn><issn>1559-0720</issn><issn>0163-4984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNp90UtPGzEQAGCrAjU8eu6NrjjQ0zZ-7to5AVFakFCR2uRsOeMJNWR3g72LlH-Pq0RqxYHDaObwzUgzQ8hnRr8xodV42WOcGDFhZQ7JPpAjppQpac3pwX_1iByn9Egpq7kRH8mIcS2UNPqIXP4M8ITrwuMqQMAWtoUPTWhD-oOpSBuMTfE8uLYP_bZwrS-a7gUbbPsitEV0fTolhyu3Tvhpn0_I4vtsPr0p7-5_3E6v7kqQkvclKOUBKJdMUW0kGG4cSAGeGu8FClMLXsGyUkANOOBL7iuJ0iEToLnS4oR83c3dxO55wNTbJiTA9dq12A3J6rysyFJlefGurIWsDec8w_M38LEbYpu3sJxpUUmpq4zGOwSxSyniym5iaFzcWkbt3x_Y6_nslzXCMptDstxxth87LBv0__z-6Bl82YGV66x7iCHZxW9OmaDMMKqrWrwC-veKig</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Yokoi, Katsuhiko</creator><creator>Uthus, Eric O</creator><creator>Nielsen, Forrest H</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7QP</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Nickel deficiency diminishes sperm quantity and movement in rats</title><author>Yokoi, Katsuhiko ; Uthus, Eric O ; Nielsen, Forrest H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-c55dcc024150894c929ac43cd09dd3e397326cb65c09cac2b2d64e4ae13c82583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>animal reproduction</topic><topic>Animals</topic><topic>cyclic nucleotide-gated channels</topic><topic>deficiency diseases</topic><topic>Diet</topic><topic>dietary mineral supplements</topic><topic>Dietary Supplements</topic><topic>Drinking water</topic><topic>enzyme inhibitors</topic><topic>epididymis</topic><topic>Epididymis - cytology</topic><topic>Epididymis - drug effects</topic><topic>Epididymis - physiology</topic><topic>Experiments</topic><topic>Male</topic><topic>NG-Nitroarginine Methyl Ester - administration & dosage</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Nickel</topic><topic>Nickel - administration & dosage</topic><topic>Nickel - deficiency</topic><topic>Nickel - pharmacology</topic><topic>Nitric oxide</topic><topic>nitric oxide synthase</topic><topic>nutrient deficiencies</topic><topic>Organ Size - drug effects</topic><topic>Physiology</topic><topic>prostate gland</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reproduction</topic><topic>Rodents</topic><topic>Sodium chloride</topic><topic>Sodium Chloride, Dietary - pharmacology</topic><topic>Sperm Count</topic><topic>sperm motility</topic><topic>Sperm Motility - drug effects</topic><topic>Sperm Motility - physiology</topic><topic>spermatozoa</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>testes</topic><topic>weanlings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yokoi, Katsuhiko</creatorcontrib><creatorcontrib>Uthus, Eric O</creatorcontrib><creatorcontrib>Nielsen, Forrest H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biological trace element research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yokoi, Katsuhiko</au><au>Uthus, Eric O</au><au>Nielsen, Forrest H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nickel deficiency diminishes sperm quantity and movement in rats</atitle><jtitle>Biological trace element research</jtitle><addtitle>Biol Trace Elem Res</addtitle><date>2003</date><risdate>2003</risdate><volume>93</volume><issue>1-3</issue><spage>141</spage><epage>153</epage><pages>141-153</pages><issn>1559-0720</issn><issn>0163-4984</issn><eissn>1559-0720</eissn><eissn>0163-4984</eissn><abstract>Early studies on nickel essentiality with rats and goats indicated that nickel deprivation impaired reproductive performance. Nickel also has been found to influence cyclic nucleotide gated channels (CNG); these types of channels are important in sperm physiology. Thus, two experiments were conducted to test the hypothesis that nickel deficiency affects sperm physiology in a manner consistent with nickel having an essential function related to CNG channel functions. The experiments were factorially arranged with four treatment groups of eight weanling rats in each. In experiment 1, the treatments were supplemental dietary nickel of 0 and 1 mg/kg and N(ω)-nitro-l-arginine methyl ester (l-NAME, a nitric oxide synthase inhibitor) added to the drinking water (50 mg/100 mL) the last 3 wk of an 8-wk experiment. In experment 2, the treatments were supplemental dietary nickel at 0 and 1 mg/kg and supplemental dietary sodium chloride (NaCl) at 0 and 80 g/kg. The NaCl and l-NAME variables were included to act as stressors affecting CNG channel activity. The basal diet contained per kilogram about 27 µg of nickel and 1 g of sodium. After 8 wk in experiment 1 and 16 wk in experiment 2, urine while fasting and testes and epididymis in both experiments, and seminal vesicles and prostates in experiment 2 were harvested for analysis. Nickel deprivation significantly decreased spermatozoa motility and density in the epididymides, epididymal transit time of spermatozoa, and testes sperm production rate. Nickel deficiency also significantly decreased the weights of the seminal vesicles and prostate glands. Excessive NaCl had no effect on sperm physiology; however, it decreased prostate gland weights. The findings support the hypothesis that nickel has an essential function that possibly could affect reproductive performance in higher animals, perhaps through affecting a CNG channel function.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>12835498</pmid><doi>10.1385/bter:93:1-3:141</doi><tpages>13</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1559-0720 |
ispartof | Biological trace element research, 2003, Vol.93 (1-3), p.141-153 |
issn | 1559-0720 0163-4984 1559-0720 0163-4984 |
language | eng |
recordid | cdi_proquest_miscellaneous_815538255 |
source | Springer Nature:Jisc Collections:Springer Nature Read and Publish 2023-2025: Springer Reading List |
subjects | animal reproduction Animals cyclic nucleotide-gated channels deficiency diseases Diet dietary mineral supplements Dietary Supplements Drinking water enzyme inhibitors epididymis Epididymis - cytology Epididymis - drug effects Epididymis - physiology Experiments Male NG-Nitroarginine Methyl Ester - administration & dosage NG-Nitroarginine Methyl Ester - pharmacology Nickel Nickel - administration & dosage Nickel - deficiency Nickel - pharmacology Nitric oxide nitric oxide synthase nutrient deficiencies Organ Size - drug effects Physiology prostate gland Rats Rats, Sprague-Dawley Reproduction Rodents Sodium chloride Sodium Chloride, Dietary - pharmacology Sperm Count sperm motility Sperm Motility - drug effects Sperm Motility - physiology spermatozoa Spermatozoa - cytology Spermatozoa - drug effects Spermatozoa - physiology testes weanlings |
title | Nickel deficiency diminishes sperm quantity and movement in rats |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T02%3A22%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nickel%20deficiency%20diminishes%20sperm%20quantity%20and%20movement%20in%20rats&rft.jtitle=Biological%20trace%20element%20research&rft.au=Yokoi,%20Katsuhiko&rft.date=2003&rft.volume=93&rft.issue=1-3&rft.spage=141&rft.epage=153&rft.pages=141-153&rft.issn=1559-0720&rft.eissn=1559-0720&rft_id=info:doi/10.1385/bter:93:1-3:141&rft_dat=%3Cproquest_cross%3E1940873821%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c442t-c55dcc024150894c929ac43cd09dd3e397326cb65c09cac2b2d64e4ae13c82583%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=218364486&rft_id=info:pmid/12835498&rfr_iscdi=true |