Optimization of nisin production by Lactococcus lactis

• Published in: Applied biochemistry and biotechnology 2002, Vol.98-100 (1-9), p.775-789
• Main Authors: VESSONI PENNA, Thereza Christina, MORAES, Dante Augusto
• Format: Article
• Language: English
• Subjects: Asparagine; Asparagine - metabolism; Bacteriological Techniques - methods; Batch culture; Biological and medical sciences; Biotechnology; Cell culture; Culture Media; Dry cells; Fermentation; Fractional factorial design; Fundamental and applied biological sciences. Psychology; Lactococcus lactis; Lactococcus lactis - growth & development; Lactococcus lactis - metabolism; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Nisin; Nisin - biosynthesis; Nutrients; Optimization; Phosphates - metabolism; Potassium; Potassium phosphate; Potassium phosphates; Regression Analysis; Sucrose; Sucrose - metabolism
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:98-100:1-9:775

The production of nisin by batch culture of Lactococcus lactis ATCC 11454 in MRS broth (pH 6.5), as treated in 30 assays, that were set up by a fractional factorial design of two levels (2[4-1]), was improved. The minimum and maximum concentrations of sucrose (5.0-12.5 g/L), asparagine (7.5-75 g/L), potassium phosphate (6.0-18.0 g/L), and Tween-80 (1.0-6.6 g/L) were added to MRS broth. The best nisin activities ranged from 1.5 x 10(4) to 1.8 x 10(4) arbitrary units (AU)/mL for the maximum levels of sucrose, asparagine, and monobasic potassium phosphate, and for the minimum concentration of Tween-80. The best following proportions between nutrients were adopted as optimum for maximum specific nisin productivity of about 6.0 mg/mg of dry cell weight (related to 2.5 mg of pure nisin preparation with a specific activity of 1.0 x 10(5) AU/mL): C/N = 0.17, C/P = 0.69, N/P = 4.17 (C = sucrose, N = asparagine, P = phosphate, T = Tween-80).