Neospora caninum: In vitro culture of tachyzoites in MCF-7 human breast carcinoma cells

The development of Neospora caninum tachyzoites, an apicomplexan protozoan parasite, was studied in vitro using the human breast carcinoma cell 7 (MCF-7) as the host cell line. The extracellular NC-1 tachyzoites in MCF-7 cells were observed and counted daily for 6 consecutive days post-infection to...

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Bibliographic Details
Published in:Experimental parasitology 2010-12, Vol.126 (4), p.536-539
Main Authors: Lv, Qiang, Li, Jianhua, Gong, Pengtao, Xing, Shenyang, Zhang, Xichen
Format: Article
Language:English
Subjects:
Acridine Orange
Animals
Biological and medical sciences
Breast Neoplasms
Carcinoma, Ductal, Breast
Cell Line, Tumor
Cercopithecus aethiops
Culture
Female
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Human cancer cell line
Humans
Life cycle. Host-agent relationship. Pathogenesis
MCF-7
Microscopy, Confocal
Neospora - growth & development
Neospora - isolation & purification
Neospora caninum
Protozoa
Tachyzoites
Vero Cells
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Summary:The development of Neospora caninum tachyzoites, an apicomplexan protozoan parasite, was studied in vitro using the human breast carcinoma cell 7 (MCF-7) as the host cell line. The extracellular NC-1 tachyzoites in MCF-7 cells were observed and counted daily for 6 consecutive days post-infection to establish the growth curve. The intracellular parasites were observed by acridine orange staining using Laser scanning confocal microscope. The results indicated that NC-1 tachyzoites invaded MCF-7 cells and multiplied intracellularly. The number of extracellular NC-1 tachyzoites started to increase rapidly around day 3 and reached the maximum number around day 4. Results from the present study suggested that MCF-7 cells were susceptible to NC-1 tachyzoites and could be used as an alternative cell line for in vitro studies.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2010.06.006