Enhancement of rhamnoplipid production in residual soybean oil by an isolated strain of Pseudomonas aeruginosa

In the present work, the production of rhamnolipid from residual soybean oil (RSO) from food frying facilities was studied using a strain of Pseudomonas aeruginosa of contaminated lagoon, isolated from a hydrocarbon contaminated soil. The optimization of RSO, ammonium nitrate, and brewery residual y...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2007-04, Vol.137-140 (1-12), p.463-470
Main Authors: de Lima, C J B, França, F P, Sérvulo, E F C, Resende, M M, Cardoso, V L
Format: Article
Language:English
Subjects:
Ammonium
Ammonium nitrate
Bacteria
Bioreactors - microbiology
Breweries
Cell Culture Techniques - methods
Fermentation
Glycolipids - metabolism
Lagoons
Pilot Projects
Pseudomonas aeruginosa
Pseudomonas aeruginosa - classification
Pseudomonas aeruginosa - metabolism
Soil contamination
Soybean oil
Soybean Oil - metabolism
Soybeans
Species Specificity
Studies
Surface tension
Surface-Active Agents - metabolism
Yeasts
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Summary:In the present work, the production of rhamnolipid from residual soybean oil (RSO) from food frying facilities was studied using a strain of Pseudomonas aeruginosa of contaminated lagoon, isolated from a hydrocarbon contaminated soil. The optimization of RSO, ammonium nitrate, and brewery residual yeast concentrations was accomplished by a central composite experimental design and surface response analysis. The experiments were performed in 500-mL Erlenmeyer flasks containing 50 mL of mineral medium, at 170 rpm and 30 +/- 1 degrees C, for a 48-h fermentation period. Rhamnolipid production has been monitored by measurements of surface tension, rhamnose concentration, and emulsifying activity. The best-planned results, located on the central point, have corresponded to 22 g/L of RSO, 5.625 g/L of NH(4)NO(3), and 11.5 g/L of brewery yeast. At the maximum point the values for rhamnose and emulsifying index were 2.2 g/L and 100%, respectively.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-007-9072-0