Enhanced virus removal by flocculation and microfiltration

In this work we have investigated the feasibility of virus clearance by flocculation and tangential flow microfiltration. Chinese hamster ovary cell feed streams were spiked with minute virus of mice and then flocculated using cationic polyelectrolytes prior to tangential flow microfiltration. Our r...

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Bibliographic Details
Published in:Biotechnology and bioprocess engineering 2002-02, Vol.7 (1), p.6-9
Main Authors: Han, Binbing, Carlson, Jonathan O., Powers, Scott M., Wickramasinghe, S. Ranil
Format: Article
Language:English
Subjects:
Biopharmaceuticals
Bioreactors
Cells
Clearances
Flocculation
Hurricanes
Meteorological satellites
Microfiltration
Minute virus of mice
Ovaries
Particle size
Polyelectrolytes
Pore size
Production costs
Viruses
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Summary:In this work we have investigated the feasibility of virus clearance by flocculation and tangential flow microfiltration. Chinese hamster ovary cell feed streams were spiked with minute virus of mice and then flocculated using cationic polyelectrolytes prior to tangential flow microfiltration. Our results indicate that flocculation prior to microfiltration leads to more than 100 fold clearance of minute virus of mice particles in the permeate. Today, validation of virus clearance is a major concern in the manufacture of biopharmaceutical products. Frequently new unit operations are added simply to validate virus clearance thus increasing the manufacturing cost. The results obtained here suggest that virus clearance can be obtained during tangential flow microfiltration. Since tangential flow microfiltration is frequently used for bioreactor harvesting this could be a low cost method to validate virus clearance.
ISSN:1226-8372
1976-3816
DOI:10.1007/BF02935872