Lipids and DNA oxidation in Staphylococcus aureus as a consequence of oxidative stress generated by ciprofloxacin

Ciprofloxacin induced an increment of reactive oxygen species in sensitive strains of Staphylococcus aureus leading to oxidative stress detected by chemiluminescence while resistant strains did not suffer such stress. Oxidation of lipids was performed by employing thiobarbituric acid reaction to det...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2006-04, Vol.285 (1-2), p.29-34
Main Authors: Becerra, María Cecilia, Páez, Paulina Laura, Laróvere, Laura E, Albesa, Inés
Format: Article
Language:English
Subjects:
Anti-Infective Agents - pharmacology
Antibiotics
Bacteria
Chemiluminescence
Ciprofloxacin - pharmacology
Deoxyribonucleic acid
DNA
DNA Damage - drug effects
DNA, Bacterial - drug effects
DNA, Bacterial - metabolism
Drug Resistance
Humans
Lipid Peroxidation - drug effects
Lipids
Liquid chromatography
Luminescent Measurements
Malondialdehyde - metabolism
Oxidation
Oxidative Stress
Peroxidation
Reactive Oxygen Species - pharmacology
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - metabolism
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Summary:Ciprofloxacin induced an increment of reactive oxygen species in sensitive strains of Staphylococcus aureus leading to oxidative stress detected by chemiluminescence while resistant strains did not suffer such stress. Oxidation of lipids was performed by employing thiobarbituric acid reaction to detect the formation of the amplified intermediate between reactive species oxygen and cytoplasmic macromolecules, namely malondialdehyde (MDA). The sensitive strain presented higher peroxidation of lipids than the resistant strain. The oxidative consequence for DNA was investigated by means of bacteria incubation with ciprofloxacin and posterior extraction of DNA, which was studied by high performance liquid chromatography (HPLC). Sensitive S. aureus ATCC 29213 showed an increase of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) respect controls without antibiotic; there was evident increase of the ratio between 8-oxodG and deoxyguanosine (dG) as a consequence of oxidation of dG to 8-oxodG considered the major DNA marker of oxidative stress. The resistant strain showed low oxidation of DNA and the analysis of 8-oxodG/dG ratio indicated lesser formation of 8-oxodG than S. aureus ATCC 29213.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-005-9051-0