Expression profiling of genes associated with regulatory functions of T-cell subsets in Marek's disease virus-infected chickens

The environment of tumours caused by Marek's disease virus (MDV) in chickens has been shown to have an immunoregulatory phenotype. The objective of the present study was to examine the expression of key T-regulatory markers during various stages of MDV pathogenesis. Specific-pathogen free (SPF)...

Full description

Saved in:
Bibliographic Details
Published in:Avian pathology 2010-10, Vol.39 (5), p.367-373
Main Authors: Parvizi, Payvand, Andrzejewski, Katelyn, Read, Leah R., Behboudi, Shahriar, Sharif, Shayan
Format: Article
Language:English
Subjects:
Animals
Biomarkers
CD4-Positive T-Lymphocytes - metabolism
CD8-Positive T-Lymphocytes - metabolism
Cells
Chickens
Gene expression
Gene Expression Profiling
Gene Expression Regulation - immunology
Genotype & phenotype
Marek Disease - metabolism
Pathogenesis
Poultry
Spleen - cytology
Viral infections
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The environment of tumours caused by Marek's disease virus (MDV) in chickens has been shown to have an immunoregulatory phenotype. The objective of the present study was to examine the expression of key T-regulatory markers during various stages of MDV pathogenesis. Specific-pathogen free (SPF) as well as major histocompatibility complex-defined chickens were infected with the RB1B and JM-16 strains of MDV, respectively. CD4 + and CD8 + T cells from the spleens of infected as well as age-matched controls were sorted by flow cytometry at 4, 10, and 21 days post infection (d.p.i.). The expression of molecules such as CTLA-4, IL-2aR (CD25), PD-1 and PDL-1 was quantified by real-time, quantitative, reverse-transcription polymerase chain reaction. There was an up-regulation of CTLA-4 in CD4 + T cells at 4 d.p.i. The expression of PD-1 was also up-regulated in the CD4 + T-cell subset of SPF birds at 21 d.p.i. Furthermore, the expression of PD-1 was enhanced in CD4 + and CD8 + T cells of genetically susceptible chickens, linking this molecule to susceptibility to disease. The expression of CD25 was down-regulated in both SPF and genetically defined birds after infection. This may be a mechanism through which the virus exerts its immunosuppressive effects. In conclusion, the results of the present study provide more insight into immunomodulatory processes that occur in the lymphoid tissues of MDV-infected chickens.
ISSN:0307-9457
1465-3338
DOI:10.1080/03079457.2010.508776