Inactivation of protein kinase C in rat liver during late hypoglycemic phase of sepsis

Changes in protein kinase C (PKC) (calcium- and phospholipid-dependent protein kinase) activity in rat liver during different metabolic phases of sepsis were studied. Sepsis was induced by cecal ligation and puncture (CLP). Experiments were divided into three groups: control, early sepsis, and late...

Full description

Saved in:
Bibliographic Details
Published in:Molecular and cellular biochemistry 1998-04, Vol.181 (1-2), p.181-189
Main Authors: Hsu, C, Jao, H C, Yang, S L, Hsu, H K, Liu, M S
Format: Article
Language:English
Subjects:
Ammonium
Animals
ATP
Cell Membrane - enzymology
Cellulose
Cytosol - enzymology
Fractionation
Hypoglycemia - enzymology
Inactivation
Kinases
Kinetics
Liver - enzymology
Magnesium Chloride - pharmacology
Male
Protein Kinase C - isolation & purification
Protein Kinase C - metabolism
Proteins
Rats
Rats, Sprague-Dawley
Sepsis
Sepsis - enzymology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Changes in protein kinase C (PKC) (calcium- and phospholipid-dependent protein kinase) activity in rat liver during different metabolic phases of sepsis were studied. Sepsis was induced by cecal ligation and puncture (CLP). Experiments were divided into three groups: control, early sepsis, and late sepsis. Early and late sepsis refers to those animals sacrificed at 9 and 18 h, respectively, after CLP. Hepatic PKC was extracted and partially purified by ammonium sulfate fractionation and DEAE-cellulose chromatography. PKC activity was assayed based on the rate of incorporation of 32p from [gamma-32P]ATP into histone. The results show that during early sepsis, both membrane-associated and cytosolic PKC activities remained relatively unaltered. During late sepsis, membrane-associated PKC was unaffected while cytosolic PKC activity was decreased by 19.5-34.4%. Kinetic analysis of the data on cytosolic PKC during late phase of sepsis reveals that the Vmax values for ATP, histone, Ca2+, phosphatidylserine, and diacylglycerol were decreased by 23.4, 22.1, 19.5, 25, and 34.4%, respectively, with no changes in their Km values. These data indicate that cytosolic PKC activity was inactivated in rat liver during late hypoglycemic phase of sepsis. Since PKC-mediated phosphorylation plays an important role in regulating hepatic glucose metabolism, an inactivation of cytosolic PKC may contribute to the development of hypoglycemia during late phase of sepsis.
ISSN:0300-8177
1573-4919
DOI:10.1023/A:1006853106320