Characteristics of zero-quantum correlation spectroscopy in MAS NMR experiments

• Published in: Journal of magnetic resonance (1997) 2010-12, Vol.207 (2), p.197-205
• Main Authors: Köneke, Stephanie G., van Beek, Jacco D., Ernst, Matthias, Meier, Beat H.
• Format: Article
• Language: English
• Subjects: Analogies; Arginine - chemistry; Carbon Monoxide - chemistry; Carbon Radioisotopes; Coherence; Correlation; Couplings; Decoupling; Electronics; Magic-angle spinning; Magnetic Resonance Spectroscopy - methods; Molecular Conformation; Multi-quantum coherence; Nitrogen Radioisotopes; NMR; Nuclear magnetic resonance; Phenylalanine - chemistry; Quantum Theory; Recoupling; Spectra; Spectral resolution; Spectroscopy; Ubiquitin - chemistry; Valine - chemistry; Zero-quantum coherence
• ISSN: 1090-7807; 1096-0856
• DOI: 10.1016/j.jmr.2010.08.020

Zero-quantum coherence generation and reconversion in magic-angle spinning solid-state NMR is analyzed. Two methods are discussed based on implementations using symmetry-based pulse sequences that utilize either isotropic J couplings or dipolar couplings. In either case, the decoupling of abundant proton spins plays a crucial role for the efficiency of the zero-quantum generation. We present optimized sequences for measuring zero-quantum single-quantum correlation spectra in solids, achieving an efficiency of 50% in ubiquitin. The advantages and disadvantages of zero-quantum single-quantum over single-quantum single-quantum correlation spectroscopy are explored, and similarities and differences with double-quantum single-quantum correlation spectroscopy are discussed. Finally, possible application of zero-quantum single-quantum experiments to polypeptides, where it can lead to better spectral resolution is investigated using ubiquitin, where we find high efficiency and high selectivity, but also increased line widths in the MQ dimension.