Dielectric Coagulometry: A New Approach To Estimate Venous Thrombosis Risk

We present dielectric coagulometry as a new technique to estimate the risk of venous thrombosis by measuring the permittivity change associated with the blood coagulation process. The method was first tested for a simple system of animal erythrocytes suspended in fibrinogen solution, where the coagu...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2010-12, Vol.82 (23), p.9769-9774
Main Authors: Hayashi, Yoshihito, Katsumoto, Yoichi, Omori, Shinji, Yasuda, Akio, Asami, Koji, Kaibara, Makoto, Uchimura, Isao
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood and lymphatic vessels
Blood Coagulation
Cardiology. Vascular system
Cattle
Coagulation
Dielectric Spectroscopy - methods
Dielectrics
Erythrocytes
Erythrocytes - chemistry
Erythrocytes - metabolism
Estimating techniques
Fibrinogen - metabolism
Horses
Humans
Medical sciences
Models, Theoretical
Rabbits
Rheology - methods
Risk Factors
Thrombin - metabolism
Thrombosis
Venous Thrombosis - blood
Venous Thrombosis - diagnosis
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Summary:We present dielectric coagulometry as a new technique to estimate the risk of venous thrombosis by measuring the permittivity change associated with the blood coagulation process. The method was first tested for a simple system of animal erythrocytes suspended in fibrinogen solution, where the coagulation rate was controlled by changing the amount of thrombin added to the suspension. Second, the method was applied to a more realistic system of human whole blood, and the inherent coagulation process was monitored without artificial acceleration by a coagulation initiator. The time dependence of the permittivity at a frequency around 1 MHz showed a distinct peak at a time that corresponds to the clotting time. Our theoretical modeling revealed that the evolution of heterogeneity and the sedimentation in the system cause the peak of the permittivity.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac101927n