Improved nude mouse models for green fluorescence human endometriosis

• Published in: The journal of obstetrics and gynaecology research 2010-12, Vol.36 (6), p.1214-1221
• Main Authors: Liu, Bin, Wang, Ning-ning, Wang, Zi-lian, Hong, Shan-shan, Li, Jian-tao, Ding, Hui-juan, Pan, Qiu-hui, Dong, Yu, Zhou, Can-quan, Zhuang, Guang-lun
• Format: Article
• Language: English
• Subjects: Ad-EGFP; Adenoviridae; Adult; Animals; Disease Models, Animal; Endometriosis; Female; Genetic Vectors; Green Fluorescent Proteins - genetics; Humans; Injections, Subcutaneous; Mice; Mice, Inbred BALB C; Mice, Nude; noninvasive animal mode; nude mice; Transfection; Young Adult
• ISSN: 1341-8076; 1447-0756
• DOI: 10.1111/j.1447-0756.2010.01345.x

Aim:  To establish an improved noninvasive fluorescent animal model for endometriosis. Material and Methods:  Adenovirus encoding enhanced green fluorescent protein (Ad‐eGFP) was used to transfect primary culture endometrial glandular cells and stromal cells (purified cell transfection and mixed injection, Group 1) as well as endometrial fragments (tissues transfection and injection, Group 2). Transfection results were compared between the cells and tissues in vitro. The GFP‐transfected cells suspension of Group 1 or endometrial fragments of Group 2, with similar weight, were injected into nude mice subcutaneously and noninvasively observed every 5 days until day 15 (Subgroup 1, N = 5), day 20 (Subgroup 2, N = 5) or day 25 (Subgroup 3, N = 11). The positive rates and duration times of the fluorescent lesions were calculated. Results:  After 18 h of incubation, glandular cells and stromal cells all had higher GFP‐positive rates. In vivo imaging showed that the GFP positive rates of Group 1 were significantly higher than those of Group 2. The fluorescent‐positive durations of Groups 1 and 2 were 23.636 ± 4.523 days and 5.909 ± 5.394 days, respectively (P