Determination of trace elements in bone by two-jet plasma atomic emission spectrometry

• Published in: Analytical and bioanalytical chemistry 2008-05, Vol.391 (2), p.687-693
• Main Authors: Zaksas, Natalia P, Sultangazieva, Toktobubu T, Gerasimov, Vladimir A
• Format: Article
• Language: English
• Subjects: Analytic Sample Preparation Methods; Analytical Chemistry; Biochemistry; Bone; Bone and Bones - chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Food Science; Graphite powder; hydroxyapatite; Laboratory Medicine; Limit of Detection; Matrix effect; Monitoring/Environmental Analysis; Original Paper; Spectrophotometry, Atomic - instrumentation; Spectrophotometry, Atomic - methods; Trace Elements - analysis; Two-jet plasma
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-008-2050-8

This paper describes an analytical method for trace element determination in bone tissues. The study of the influence of the bone matrix showed that the addition of 25% ground bone to graphite powder with introduced impurities did not affect the analytical signal of elements in the spectral excitation in a two-jet plasma. On basis of these investigations a method for direct multielement analysis of bone tissues was suggested. The sample preparation procedure consisted in mixing powdered bone (particle size 30 μm or less) with a spectroscopic buffer (graphite powder plus NaCl) in ratio 1:3 or to a greater extent depending on the analyte concentration. Reference samples based on graphite powder were used for construction of calibration curves. The NaCl concentration in analyzed and calibration samples was 15 wt%. The effect of particle size was revealed from the determination of Ba, Sr, and Mg. To eliminate this effect, treatment of the samples with nitric acid was proposed. The validation of the technique was confirmed by comparison of the analysis results of a bone sample with those obtained by inductively coupled plasma atomic emission spectrometry after wet acid digestion. The limits of detection estimated for 20 elements were the following (μg g⁻¹): 0.1 (Ag), 1.0 (Al), 1.0 (Ba), 0.1 (Be), 1.2 (Bi), 0.4 (Cd), 1.0 (Co), 0.2 (Cu), 0.6 (Cr), 1.9 (In), 2 (Fe), 0.3 (Ga), 0.4 (Mn), 0.4 (Mo), 0.7 (Ni), 1.0 (Pb), 0.7 (Sn), 0.8 (Tl), 5 (Sr), 1.0 (Zn).