Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations

Isolated rat brain tubulin can be repolymerized in vitro in solutions containing adenosine triphosphate or guanosine triphosphate, magnesium ions, and a good calcium chelator. The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and sug...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1972-09, Vol.177 (4054), p.1104-1105
Main Author: Weisenberg, Richard C.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - pharmacology
Animals
Brain - metabolism
Calcium
Calcium - pharmacology
Calcium Chloride - pharmacology
Chelating Agents - pharmacology
Edetic Acid - pharmacology
Guanosine Triphosphate - pharmacology
Homogenization
Imino acids
In Vitro Techniques
Magnesium Sulfate - pharmacology
Microtubules
Microtubules - metabolism
Polymerization
Polymers
Quaternary ammonium compounds
Rats
RNA
Sodium
Sulfates
Tissue Extracts - metabolism
Vitamin A
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Summary:Isolated rat brain tubulin can be repolymerized in vitro in solutions containing adenosine triphosphate or guanosine triphosphate, magnesium ions, and a good calcium chelator. The extreme sensitivity of tubulin to calcium ions explains the failure of previous efforts to obtain polymerization and suggests a possible mechanism for regulation of microtubule polymerization in vivo.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.177.4054.1104