Binding of Concanavalin A to Rat, Normal Human, and Chronic Lymphatic Leukemia Lymphocytes

This study was aimed to find a correlation between the extent of concanavalin A (Con A) binding to lymphocytes and the blastogenie effect of the lectin on these cells. 63Ni-labeled Con A was used in the lectin-binding experiments. Binding of Con A to a small fraction (approximately 3%) of Con A bind...

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Bibliographic Details
Published in:Blood 1972-09, Vol.40 (3), p.311-316
Main Authors: Novogrodsky, Abraham, Biniaminov, Miriam, Ramot, Bracha, Katchalski, Ephraim
Format: Article
Language:English
Subjects:
Animals
Binding Sites
Cells, Cultured
Concanavalin A
DNA - biosynthesis
Humans
Lectins
Leukemia, Lymphoid - immunology
Lymph Nodes - cytology
Lymph Nodes - immunology
Lymphocyte Activation
Lymphocytes - immunology
Male
Niobium
Rats
RNA - biosynthesis
Thymidine - metabolism
Tritium
Uridine - metabolism
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Summary:This study was aimed to find a correlation between the extent of concanavalin A (Con A) binding to lymphocytes and the blastogenie effect of the lectin on these cells. 63Ni-labeled Con A was used in the lectin-binding experiments. Binding of Con A to a small fraction (approximately 3%) of Con A binding sites in rat lymphocytes is required for the maximal stimulation of RNA synthesis. Binding of Con A to additional sites resulted in a less stimulatory effect. Lymphocytes from patients with chronic lymphatic leukemia (CLL) are impaired in their blastogenie response to Con A. Maximal stimulation of DNA synthesis induced by Con A in CLL lymphocytes is attained by treatment of the cells with the lectin at concentrations that are smaller than those required for the induction of maximal DNA synthesis in normal lymphocytes. The number of Con A binding sites in CLL lymphocyte is smaller than in normal lymphocyte.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V40.3.311.311