Ventilation strategy and its influence on the functional performance of lung grafts in an experimental model of single lung transplantation using non-heart-beating donors

• Published in: Jornal brasileiro de pneumologia 2010-09, Vol.36 (5), p.554-561
• Main Authors: Felix, Elaine Aparecida, Andrade, Cristiano Feijó, Cardoso, Paulo Francisco Guerreiro, Thiesen, Gabriela Cury, Antonio, Ana Carolina Peçanha, Martins, Lucas Krieger, Tonietto, Tiago Antonio
• Format: Article
• Language: eng ; por
• Subjects: Animals; Dogs; Lung - pathology; Lung - physiopathology; Lung Transplantation - methods; Lung Transplantation - physiology; Models, Animal; Organ Preservation; Random Allocation; Respiration, Artificial - methods
• ISSN: 1806-3756
• DOI: 10.1590/S1806-37132010000500006

To compare the influence of two different ventilation strategies-volume-controlled ventilation (VCV) and pressure-controlled ventilation (PCV)-on the functional performance of lung grafts in a canine model of unilateral left lung transplantation using donor lungs harvested after three hours of normothermic cardiocirculatory arrest under mechanical ventilation. The study comprised 40 mongrel dogs, randomized into two groups: VCV and PCV. Of the 20 recipients, 5 did not survive the transplant, and 5 died before the end of the post-transplant assessment period. The remaining 10 survivors (5 in each group) were evaluated for 360 min after lung transplantation. The functional performance of the grafts was evaluated regarding respiratory mechanics, gas exchange, and lung graft histology. There were no significant differences between the groups regarding respiratory mechanics (peak inspiratory pressure, plateau pressure, mean airway pressure, dynamic compliance, and static compliance) or gas exchange variables (PaO2, venous oxygen tension, PaCO2, venous carbon dioxide tension, and the arterial-venous oxygen content difference). The histopathological findings were consistent with nonspecific acute lung injury and did not differ between the groups. This model of lung transplantation showed that the functional performance of lung grafts was not influenced by the ventilation strategy employed during the first six hours after reperfusion.