Hematopoietic differentiation from human ESCs as a model for developmental studies and future clinical translations. Invited review following the FEBS Anniversary Prize received on 5 July 2009 at the 34th FEBS Congress in Prague

Human embryonic stem cells (hESCs) and induced pluripotent stem cells are excellent models for the study of embryonic hematopoiesis in vitro, aiding the design of new differentiation models that may be applicable to cell-replacement therapies. Adult and fetal hematopoietic stem cells are currently b...

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Bibliographic Details
Published in:The FEBS journal 2010-12, Vol.277 (24), p.5014-5025
Main Authors: Moreno-Gimeno, Inmaculada, Ledran, Maria H, Lako, Majlinda
Format: Article
Language:English
Subjects:
Animals
Awards and Prizes
Biochemistry
blood cells
Bone Marrow Cells - cytology
Cell Differentiation
Cell Lineage
Comparative studies
Developmental biology
Embryonic Stem Cells - cytology
engraftment
hematopoiesis
hematopoietic differentiation
hematopoietic ontogeny
hematopoietic progenitors
hESC and iPSC therapeutic applications
human embryonic stem cells
Humans
induced pluripotent stem cells
Models, Biological
SCID repopulating cells
Stem cells
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Summary:Human embryonic stem cells (hESCs) and induced pluripotent stem cells are excellent models for the study of embryonic hematopoiesis in vitro, aiding the design of new differentiation models that may be applicable to cell-replacement therapies. Adult and fetal hematopoietic stem cells are currently being used in biomedical applications; however, the latest advances in regenerative medicine and stem cell biology suggest that hESC-derived hematopoietic stem cells are an outstanding tool for enhancing immunotherapy and treatments for blood disorders and cancer, for example. In this review, we compare various methods used for inducing in vitro hematopoietic differentiation from hESCs, based on co-culture with stromal cells or formation of embryoid bodies, and analyse their ability to give rise to hematopoietic precursors, with emphasis on their engraftment potential as a measure of their functionality in vivo.
ISSN:1742-464X
1742-4658
DOI:10.1111/j.1742-4658.2010.07926.x