THE HISTOCHEMICAL DEMONSTRATION OF ACID PHOSPHATASE BY A POST-INCUBATION COUPLING TECHNIQUE

The procedure for the histochemical demonstration of acid phosphatase by a post-incubation coupling technique using sodium 6-benzoyl-2-naphthyl phosphate is described. The distribution of activity in the tissues of the rat is presented. Enzymatic activity was generally confined to the cytoplasm of e...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1955-11, Vol.3 (6), p.455-470
Main Authors: RUTENBURG, ALEXANDER M, SELIGMAN, ARNOLD M
Format: Article
Language:English
Subjects:
Acid Phosphatase
Old Medline
Phosphoric Monoester Hydrolases - analysis
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Summary:The procedure for the histochemical demonstration of acid phosphatase by a post-incubation coupling technique using sodium 6-benzoyl-2-naphthyl phosphate is described. The distribution of activity in the tissues of the rat is presented. Enzymatic activity was generally confined to the cytoplasm of epithelial cells. Prostate, liver, spleen, ovary and kidney showed the greatest acid phosphatase activity.
ISSN:0022-1554
1551-5044
DOI:10.1177/3.6.455