TUNEL as a Test for Sperm DNA Damage in the Evaluation of Male Infertility

Objectives To standardize the TUNEL assay by establishing inter- and intraobserver variability, interassay variability, cutoff values, sensitivity and specificity of the assay, and studying the distribution of the DNA damage in a population of infertile men referred to a clinical andrology laborator...

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Bibliographic Details
Published in:Urology (Ridgewood, N.J.) N.J.), 2010-12, Vol.76 (6), p.1380-1386
Main Authors: Sharma, Rakesh K, Sabanegh, Edmund, Mahfouz, Reda, Gupta, Sajal, Thiyagarajan, Aparna, Agarwal, Ashok
Format: Article
Language:English
Subjects:
Adult
Area Under Curve
Biological and medical sciences
Birth control
DNA Damage
DNA Fragmentation
Gynecology. Andrology. Obstetrics
Humans
In Situ Nick-End Labeling
Infertility, Male - diagnosis
Male
Medical sciences
Nephrology. Urinary tract diseases
Observer Variation
Predictive Value of Tests
Reference Standards
ROC Curve
Sensitivity and Specificity
Spermatozoa - chemistry
Spermatozoa - pathology
Sterility. Assisted procreation
Urology
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Summary:Objectives To standardize the TUNEL assay by establishing inter- and intraobserver variability, interassay variability, cutoff values, sensitivity and specificity of the assay, and studying the distribution of the DNA damage in a population of infertile men referred to a clinical andrology laboratory. Methods Seminal ejaculates from 25 healthy male volunteers (controls) and 194 infertile men (with male factor infertility) referred to an andrology laboratory were examined for DNA damage by TUNEL assay using flow cytometric analysis. Results Both the inter- and intraobserver variability and interassay variability was small (
ISSN:0090-4295
1527-9995
DOI:10.1016/j.urology.2010.04.036