Proteomic-based approach for biomarkers discovery in early detection of invasive urothelial carcinoma

• Published in: Proteomics. Clinical applications 2008-01, Vol.2 (1), p.78-89
• Main Authors: Li, Junyu, Abraham, Shaji, Cheng, Liang, Witzmann, Frank A., Koch, Michael, Xie, Jun, Rahman, Munazzah, Mohammed, Sulma I.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biomarker discovery; Bladder; Diverse techniques; Fundamental and applied biological sciences. Psychology; Molecular and cellular biology; Urothelial carcinoma
• ISSN: 1862-8346; 1862-8354
• DOI: 10.1002/prca.200780027

Identification of reliable non‐invasive markers for the detection of invasive phenotype of urothelial carcinoma is needed. This study characterizes and compares protein expression profiles of adjacent non‐neoplastic urothelium and invasive urothelial carcinoma to identify biomarkers for early detection of de novo bladder cancer. Differences in protein expression between adjacent non‐neoplastic and high‐grade, stage T4, grade 3 invasive urothelial carcinoma tissues were investigated using 2‐DE, MALDI‐TOF‐MS, and data processing. Ingenuity Pathway Analysis (IPA) was applied to examine the biological mechanisms represented by the altered proteins. The 2‐DE of the adjacent non‐neoplastic urothelium and invasive urothelial carcinoma showed reproducibly similar proteomic mapping for each group distinguishing adjacent non‐neoplastic urothelium from invasive urothelial carcinoma. Twenty‐one proteins were altered in expression and one of these proteins, Choroideremia‐like protein (CHML) was significantly overexpressed (p