Dispersal of Rabbit Lung into Individual Viable Cells: A New Model for the Study of Lung Metabolism

This new enzymatic method disperses rabbit lung into morphologically intact, viable individual cells. The scattered cells constitute more than 50 percent of the original tissue. At least 90 percent of the cells exclude trypan blue from the nucleus. The dispersed lung cells consumed 6.2 microliters o...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1972-12, Vol.178 (4066), p.1209-1210
Main Authors: Gould, Kenneth G., Clements, John A., Jones, Albert L., Felts, James M.
Format: Article
Language:English
Subjects:
Animals
Blood cells
Carbon Isotopes
Catalase - metabolism
Cells
Cells, Cultured
Deoxyribonucleases - metabolism
Enzymes
Insect larvae
Larval development
Lung - cytology
Lung - metabolism
Lung morphology
Lungs
Mast cells
Methods
Microbial Collagenase - metabolism
Microscopy, Electron
Microscopy, Electron, Scanning
Oxygen
Oxygen Consumption
Palmitic Acids - metabolism
Pancreatic Elastase - metabolism
Papain - metabolism
Phosphatidylcholines - biosynthesis
Pronase - metabolism
Pulmonary alveoli
Rabbits
Staining and Labeling
Trypan Blue
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Summary:This new enzymatic method disperses rabbit lung into morphologically intact, viable individual cells. The scattered cells constitute more than 50 percent of the original tissue. At least 90 percent of the cells exclude trypan blue from the nucleus. The dispersed lung cells consumed 6.2 microliters of oxygen per hour per milligram, dry weight. They incorporated [1-$^{14}$C]palmitate into lecithin.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.178.4066.1209