THE PYRIMIDINE PART OF THIAMINE DECOMPOSITION PRODUCT BY BACTERIAL THIAMINASE I

Although the detection of Dragendorff-positive spot on paper chromatogram due to the pyrimidine compound formed from thiamine by bacterial thiaminase I had failed thus far, it was shown in the present experiment that the crude crystalline pyrimidine compound (Pm-X⋅HCl) isolated from the thiamine rea...

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Bibliographic Details
Published in:Journal of vitaminology 1956, Vol.2(2), pp.95-101
Main Authors: MURATA, KIKU, MORITA, JUNKO
Format: Article
Language:English
Subjects:
Alkyl and Aryl Transferases
Enzymes
Old Medline
Pyrimidines - analysis
Thiamine
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Summary:Although the detection of Dragendorff-positive spot on paper chromatogram due to the pyrimidine compound formed from thiamine by bacterial thiaminase I had failed thus far, it was shown in the present experiment that the crude crystalline pyrimidine compound (Pm-X⋅HCl) isolated from the thiamine reaction products by the bacterial thiaminase I gave a Dragendorff-positive spot at RF 0.06 in contrast with RF 0.59 of thiamine, when n-butanol methanol-water (4:2:2) was used as the solvent system for the development of paper chromatography. The absorption maximum of Pm-X·HCl extracted by 0.001N hydrochloric acid from a paper strip around RF 0.06 of the paper chromatogram was found at 250mμ, and the form of the absorption spectrum of the substance differed somewhat from that of thiamine when measured at the same pH. Pm-X was found to promote the growth of BKA, similarly to thiamine as well as other pyrimidine compounds having an amino group on the C4-position of pyrimidine. These findings have led to the conclusion that the isolated pyrimidine compound consisted certainly of the pyrimidine moiety of thiamine and possibly of a base, the nature of which remains to be settled.
ISSN:0022-5398
DOI:10.5925/jnsv1954.2.95