Production of Tropane Alkaloids during De-differentiation of Scopolia parviflora Calli

The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2...

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Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2010-02, Vol.73 (2), p.147-150
Main Authors: Kim, Yong Duck, Kang, Seung Mi, Min, Ji Yun, Choi, Won Kyun, Jeong, Mi Jin, Karigar, Chandrakant. S, Choi, Myung Suk
Format: Article
Language:English
Subjects:
Korea
Molecular Structure
Plant Roots - chemistry
Scopolamine Hydrobromide - biosynthesis
Scopolia - chemistry
Scopolia - growth & development
Tropanes - chemistry
Tropanes - isolation & purification
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Summary:The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of 1 was enhanced in calli undergoing differentiation to shoot after 60 days of culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6β-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids, was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli.
ISSN:0163-3864
1520-6025
DOI:10.1021/np900525n