Early activation does not translate into effector differentiation of peripheral CD8T cells during the acute phase of Kawasaki disease

Early diagnosis of acute Kawasaki disease (KD), lying in the spectrum between infectious and autoimmune diseases, can be difficult. To clarify the role of peripheral CD8T cells in KD, we examined their activation, proliferation, maturation, and effector function by four-color flow cytometry. Compare...

Full description

Saved in:
Bibliographic Details
Published in:Cellular immunology 2010, Vol.265 (1), p.57-64
Main Authors: Ehara, Hiroshi, Kiyohara, Koji, Izumisawa, Yasuhiro, Ito, Taichi
Format: Article
Language:English
Subjects:
Adaptive Immunity - immunology
Antigens, CD - blood
Antigens, CD - immunology
Antigens, Differentiation, T-Lymphocyte - blood
Antigens, Differentiation, T-Lymphocyte - immunology
CD69 expression
CD8-Positive T-Lymphocytes - cytology
CD8-Positive T-Lymphocytes - immunology
CD8T cells
Cell Differentiation - immunology
Cell division
Child
Child, Preschool
Effector differentiation
Female
Flow Cytometry
Humans
Immunoglobulins, Intravenous - therapeutic use
Infant
Kawasaki disease
Ki-67 Antigen - immunology
Ki67
Lectins, C-Type - blood
Lectins, C-Type - immunology
Lymphocyte Activation - immunology
Male
Mucocutaneous Lymph Node Syndrome - immunology
Mucocutaneous Lymph Node Syndrome - therapy
Perforin
Perforin - immunology
Statistics, Nonparametric
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Early diagnosis of acute Kawasaki disease (KD), lying in the spectrum between infectious and autoimmune diseases, can be difficult. To clarify the role of peripheral CD8T cells in KD, we examined their activation, proliferation, maturation, and effector function by four-color flow cytometry. Compared to healthy/febrile controls, acute KD patients showed striking increase in early activation marker CD69 +CD8T cells and maturation subsets, but HLA-DR +CD8T cells representing late activation did not increase. Although Ki67 +CD8T cells reflecting ongoing cell division increased in acute KD and febrile controls, absolute numbers of CD8T cells and maturation subsets decreased in acute KD versus healthy controls. Effector cells were lower in acute than in convalescent KD. Perforin +CD8T cells, denoting cytolytic activity, were lower in KD patients versus febrile controls. CD69 +CD8T cells increase in acute KD but effector differentiation is absent. CD69 +CD8T cells could be a marker to determine disease progression, treatment response, and convalescence in acute KD.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2010.07.003