Confirmation of a Trimeric Subunit Arrangement for 2-Keto-3-deoxy-6-phosphogluconic Aldolase Using X-ray Crystallographic Methods

2-Keto-3-deoxy-6-phosphogluconate aldolase of Pseudomonas putida crystallizes from ammonium sulfate at pH 3.5 in the cubic system with unit cell dimensions of 103.1 ± 0.2 A in the space group P2 1 3 which has 12 equivalent positions. From variable equilibrium density measurements and the "salt...

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Bibliographic Details
Published in:The Journal of biological chemistry 1973-03, Vol.248 (6), p.2251-2253
Main Authors: Vandlen, R L, Ersfeld, D L, Tulinsky, A, Wood, W A
Format: Article
Language:English
Subjects:
Aldehyde-Lyases
Chemical Phenomena
Chemistry
Crystallization
Gluconates
Macromolecular Substances
Mathematics
Molecular Weight
Pyruvates
X-Ray Diffraction
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Summary:2-Keto-3-deoxy-6-phosphogluconate aldolase of Pseudomonas putida crystallizes from ammonium sulfate at pH 3.5 in the cubic system with unit cell dimensions of 103.1 ± 0.2 A in the space group P2 1 3 which has 12 equivalent positions. From variable equilibrium density measurements and the "salt-free" density determination, the molecular weight per unit cell was calculated to be 326,000 or 27,200 ± 2,000 per asymmetric unit, in good agreement with other chemical and physical data. Consideration of the mass per asymmetric unit, the symmetry elements of P2 1 3, and the results of chemical and physical solution studies suggest that the molecules are assemblages of trimers in the crystal with the subunits related by a 3-fold rotation axis.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)44213-0