Transcript profiling during the early development of the maize brace root via Solexa sequencing

• Published in: The FEBS journal 2011-01, Vol.278 (1), p.156-166
• Main Authors: Li, Yan‐Jie, Fu, Ya‐Ru, Huang, Jin‐Guang, Wu, Chang‐Ai, Zheng, Cheng‐Chao
• Format: Article
• Language: English
• Subjects: adventitious roots; auxins; brace root; cell walls; Corn; data collection; differential expression; early development; ethylene; gene expression; Gene Expression Profiling; gene expression regulation; Gene Expression Regulation, Plant; genes; Genetics; maize; metabolism; Plant Roots - genetics; Plant Roots - growth & development; Plant Roots - metabolism; quantitative polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction; signal transduction; Solexa sequencing; tag; tissues; transcriptome; Zea mays - genetics; Zea mays - growth & development; Zea mays - metabolism
• ISSN: 1742-464X; 1742-4658
• DOI: 10.1111/j.1742-4658.2010.07941.x

Currently, the molecular regulation mechanisms involved in the early development of maize brace root are poorly known. To gain insight into the transcriptome dynamics that are associated with its development, genome‐wide gene expression profiling was conducted by Solexa sequencing (Illumina Inc., San Diego, CA, USA). More than five million tags were generated from the stem node tissues without and with just‐emerged brace roots, including 149 524 and 178 131 clean tags in the two libraries, respectively. Of these, 82 864 (55.4%) and 91 678 (51.5%) tags were matched to the reference genes. The most differentially expressed tags with a log₂ ratio > 2 or