UV-induced fin damage in zebrafish as a system for evaluating the chemopreventive potential of broccoli and cauliflower extracts

This study applied broccoli and cauliflower extracts (whole, floret, and stem) to zebrafish larvae in parallel to receive 100 mJ/cm2 of UVB six times, and recorded their fin malformation phenotypes. Chemopreventive effects of each group, including UVB, whole-, floret-, and stem-extracts of broccoli...

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Bibliographic Details
Published in:Toxicology mechanisms and methods 2011-01, Vol.21 (1), p.63-69
Main Authors: Chen, Yau-Hung, Wen, Chi-Chung, Lin, Chun-Yun, Chou, Chi-Yuan, Yang, Zhi-Shiang, Wang, Yun-Hsin
Format: Article
Language:English
Subjects:
Animal Fins - radiation effects
Animals
Brassica - chemistry
Broccoli
cauliflower
Danio rerio
fin
Freshwater
Larva - drug effects
Plant Extracts - chemistry
Plant Extracts - pharmacology
skin cancer
Ultraviolet Rays
UVB
zebra fish
Zebrafish - embryology
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Summary:This study applied broccoli and cauliflower extracts (whole, floret, and stem) to zebrafish larvae in parallel to receive 100 mJ/cm2 of UVB six times, and recorded their fin malformation phenotypes. Chemopreventive effects of each group, including UVB, whole-, floret-, and stem-extracts of broccoli and cauliflower on fin development were evaluated using Kaplan-Meier analysis, log-rank test, and Cox proportional hazards regression. Results showed that (1) zebrafish fins in the UVB + whole broccoli extract group are 6.20~9.32-times more likely to return to normal fins than ones in the UVB only group, but fins in the UVB + whole cauliflower extract group are only 5.13~11.10-times more likely to recover, indicated that whole broccoli and cauliflower extract had similar chemopreventive ability on fin development; and (2) the broccoli stem has the highest antioxidant capacity among other groups. In conclusion, zebrafish can be used as a system for evaluating the efficacy of other UVB protective compounds.
ISSN:1537-6516
1537-6524
DOI:10.3109/15376516.2010.529191