Antimicrobial effect of photodynamic therapy using a highly pure chlorin e6

• Published in: Lasers in medical science 2010-09, Vol.25 (5), p.705-710
• Main Authors: Park, Jong-Hwan, Moon, Yeon-Hee, Bang, Iel-Soo, Kim, Yong-Chul, Kim, Soo-A, Ahn, Sang-Gun, Yoon, Jung-Hoon
• Format: Article
• Language: English
• Subjects: Antimicrobial agents; Bacteria; Bacteria - drug effects; Bacterial Load - drug effects; Dentistry; Escherichia coli; Escherichia coli - drug effects; Lasers; Medicine; Medicine & Public Health; Microbial Viability - drug effects; Optical Devices; Optics; Original Article; Photochemotherapy - methods; Photodynamic therapy; Photonics; Photosensitizing Agents - administration & dosage; Porphyrins - administration & dosage; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Quantum Optics; Salmonella enterica; Salmonella typhimurium; Salmonella typhimurium - drug effects; Staphylococcus aureus; Staphylococcus aureus - drug effects
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-010-0781-1

The aim of the present study is to evaluate the antimicrobial effect of photodynamic therapy (PDT) using a highly pure chlorin e 6 (Ce 6 ), against various pathogenic bacteria. To examine the antimicrobial effect of Ce 6 -mediated PDT against Staphylococcus aureus , Pseudomonas aeruginosa , Escherichia coli , and Salmonella enterica serovar Typhimurium, inhibition zone formation, CFU quantification, and bacterial viability were evaluated. Inhibition zone analysis showed that Ce 6 -mediated PDT is very effective to inhibit the growth of S. aureus and P. aeruginosa , but has only minor effect to E. coli and S. Typhimurium, which was dependent on the energy density of laser and dose of Ce 6 . Ce 6 -mediated PDT also nearly inhibited the colony formation of S. aureus and P. aeruginosa , and partially inhibited that of E. coli and S . Typhimurium. In addition, the number of viable bacteria decreased greatly after PDT application with LS-chlorin e6 of 10 μM and laser and energy density of 20 J/cm 2 . These results show that Ce 6 -mediated PDT can be an effective alternative for antimicrobial treatment.