Comparative Study of Two RD 114 Virus-Indicator Cell Lines, KC and KB

The chromosomes and glucose-6-phosphate dehydrogenase (Glu-6-PD) isozyme mobility patterns of human cell lines KC and KB were compared with those of KC-related strains and other strains and passage levels of H.Ep. #2 and HeLa cells. Both indicator lines, KC and KB, had human chromosomes and were het...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1973-05, Vol.50 (5), p.1129-1134
Main Authors: Nelson-Rees, Walter A., Klement, Vaclav, Peterson, Ward D., Weaver, John F.
Format: Article
Language:English
Subjects:
Avian Sarcoma Viruses
Carcinoma
Cell Line
Cell Transformation, Neoplastic
Cells - enzymology
Chromosomes
Fluorescence
Glucosephosphate Dehydrogenase
HeLa Cells
Humans
Isoenzymes
Mouth Neoplasms
Oncogenic Viruses
Retroviridae
RNA Viruses
Sex Chromosomes
Time Factors
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Summary:The chromosomes and glucose-6-phosphate dehydrogenase (Glu-6-PD) isozyme mobility patterns of human cell lines KC and KB were compared with those of KC-related strains and other strains and passage levels of H.Ep. #2 and HeLa cells. Both indicator lines, KC and KB, had human chromosomes and were heteroploid. KC cells had a mean chromosome number of 103 and KB cells of 2 strains (3 passage levels) had means of 69-76 chromosomes. KC cells had 1 or 2 Y chromosomes and B-type Glu-6-PD mobility. All KB, H.Ep. #2, and.HeLa cell cultures lacked Y chromosomes and showed A-type mobility. The parental, nontransformed strain of KC, 118 MG, and another branch of Rous sarcoma' virustransformed cells (118 MG-EH, Macintyre) differed from KC in mean number of chromosomes (118 and 69), but had Y chromosomes and type-B Glu-6-DP mobility. However, only the KC and KB cultures, but not the KC-related strains or different strains of H.Ep. #2 and HeLa cells, acted as RD 114 virus-indicator cell lines. Our results distinguished KC from KB cells, KC from its related cultures, and KB cultures from cultures of H.Ep. #2 and HeLa cells.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/50.5.1129