Serologic Characterization of a Malacosoma disstria Hübner (Lepidoptera: Lasiocampidae) Cell Line

The identity of a cell line derived from hemocytes of Malacosoma disstria was investigated serologically by using complement fixation, double diffusion, immunoelectrophoresis in agarose, and acrylamide gel electrophoresis. The M. disstria cell line antiserum gave a specific cross-reaction with its h...

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Bibliographic Details
Published in:In vitro 1973-05, Vol.8 (6), p.459-465
Main Authors: Krywienczyk, Janina, Sohi, S. S.
Format: Article
Language:English
Subjects:
Aedes
Animal cells
Animals
Antigens
Antiserum
Bombyx
Cell Line
Cell lines
Complement Fixation Tests
Cross Reactions
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Gels
Hemolymph
Immune Sera
Immunodiffusion
Immunoelectrophoresis
Insect larvae
Insecta - immunology
Larva
Larvae
Polysaccharides
Precipitins
Rabbits - immunology
Species Specificity
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Summary:The identity of a cell line derived from hemocytes of Malacosoma disstria was investigated serologically by using complement fixation, double diffusion, immunoelectrophoresis in agarose, and acrylamide gel electrophoresis. The M. disstria cell line antiserum gave a specific cross-reaction with its homologous antigen and with M. disstria larval antigen, although cross-reactions with cell lines from Aedes aegypti, Bombyx mori, and Choristoneura fumiferana and with larval antigens of B. mori and C. fumiferana were also produced. Antisera against A. aegypti and B. mori cell lines showed very similar cross-reaction with both the cell line antigens. Also, these two antisera gave a strong reaction with B. mori larval antigen, but no reaction with A. aegypti larval antigen. These tests confirm the identity of our M. disstria cell line. Also, they indicate: (a) that the A. aegypti and B. mori cell lines tested are similar, and (b) that they are closely related to B. mori, but not at all related to A. aegypti.
ISSN:0073-5655
DOI:10.1007/BF02615947