Molecular cloning and expression analysis of feline α₁-microglobulin

• Published in: Veterinary immunology and immunopathology 2011, Vol.139 (1), p.79-82
• Main Authors: Nakata, Junya, Nakahari, Aiko, Kato, Yohei, Yamachi, Nanako, Sato, Nami, Yonezawa, Tomohiro, Takahashi, Chika, Chikazawa, Seishiro, Hori, Yasutomo, Kanai, Kazutaka, Ito, Naoyuki, Higuchi, Seiichi, Hoshi, Fumio
• Format: Article
• Language: English
• Subjects: Alpha-Globulins - biosynthesis; Alpha-Globulins - genetics; alpha1-microglobulin-bikunin; Amino Acid Sequence; amino acid sequences; animal proteins; animal tissues; Animals; Base Sequence; cats; Cats - genetics; Cloning, Molecular; complementary DNA; gene expression; globulins; glycoproteins; Liver - metabolism; molecular sequence data; open reading frames; Open Reading Frames - genetics; proteinase inhibitors; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Messenger - genetics; Sequence Alignment; sequence analysis; spatial variation
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2010.08.002

Full-length cDNA that encodes feline α₁-microglobulin (Feα₁m)-bikunin was obtained from a feline liver and cloned using an oligo-capping method. The Feα₁m-bikunin cDNA was found to contain 1284 nucleotides, and Feα₁m was found to include an open reading frame encoding a polypeptide of 201 amino acids. The deduced amino acid sequence of Feα₁m showed varying amino acid identity when compared with the published sequences of the related α₁-m of other species, ranging from 71.1 to 82.1%. Feα₁m mRNA expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR analysis in the cerebrum, cerebellum, lung, heart, liver, spleen, pancreas, kidney, adrenal gland, and testicle. The highest Feα₁m mRNA level was found in the liver.