Chemical and biological investigations of Delonix regia (Bojer ex Hook.) Raf

• Published in: Acta pharmaceutica (Zagreb, Croatia) Croatia), 2010-06, Vol.60 (2), p.207-215
• Main Authors: Jahan, Israt, Rahman, Mohammad, Rahman, Mohammed, Kaisar, Mohammad, Islam, Mohammad, Wahab, Abdul, Rashid, Mohammad
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; antimicrobial activity; antimikrobno djelovanje; Benzaldehydes - chemistry; Benzaldehydes - isolation & purification; Benzaldehydes - pharmacology; brine shrimp lethality; Fabaceae - chemistry; Kanamycin - pharmacology; metanolni ekstrakt; methanolic extract; Microbial Sensitivity Tests; Pentacyclic Triterpenes - chemistry; Pentacyclic Triterpenes - isolation & purification; Pentacyclic Triterpenes - pharmacology; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Sitosterols - chemistry; Sitosterols - isolation & purification; Sitosterols - pharmacology; smrtnost morskih kozica; Stigmasterol - chemistry; Stigmasterol - isolation & purification; Stigmasterol - pharmacology
• ISSN: 1330-0075; 1846-9558
• DOI: 10.2478/v10007-010-0018-7

In this study, five compounds, lupeol (1), epilupeol (2), β-sitosterol (3), stigmasterol (4) and p-methoxybenzaldehyde (5) were isolated from the petroleum ether and dichloromethane fractions of a methanolic extract of the stem bark of Delonix regia. Antimicrobial screening of the different extracts (15 μg mm-2) was conducted by the disc diffusion method. The zones of inhibition demonstrated by the petroleum ether, carbon tetrachloride and dichloromethane fractions ranged from 9-14 mm, 11-13 mm and 9-20 mm, respectively, compared to kanamycin standard with the zone of inhibition of 20-25 mm. In brine shrimp lethality bioassay, the carbon tetrachloride soluble materials demonstrated the highest toxicity with LC50 of 0.83 μg mL-1, while petroleum ether and dichloromethane soluble partitionates of the methanolic extract revealed LC50 of 14.94 and 3.29 μg mL-1, respectively, in comparison with standard vincristine sulphate with LC50 of 0.812 μg mL-1. This is the first report on compounds separation from D. regia, their antimicrobial activity and cytotoxicity. U radu je opisana izolacija pet sastojaka petroleterske i diklormetanske frakcije metanolnog ekstrakta kore biljke Delonix regia: lupeol (1), epilupeol (2), β-sitosterol (3), stigmasterol (4) i p-metoksibenzaldehid (5). Nadalje, testirano je antimikrobno djelovanje različitih ekstrakata difuzijskom metodom na disku (15 μg mm-2). Zone inhibicije za sastojke topljive u petroleteru, tetraklormetanu i diklormetanu bile su 9-14 mm, 11-13 mm, odnosno 9-20 mm, dok je zona inhibicije standarda kanamicina bila 20-25 mm. U biološkom pokusu smrtnosti morskih kozica najveću toksičnost pokazali su spojevi topljivi u tetraklormetanu (LC50 = 0,83 μg mL-1), dok je topljivost sastojaka topljivih u petroleteru i diklormetanu bila LC50 14,94, odnosno 3,29 μg mL-1, a standarda vinkristin sulfata 0,812 μg mL-1. Ovo je prvo izvješće o izolaciji sastojaka, antimikrobnom djelovanju i citotoksičnosti biljke D. regia.