Antigenicity of Fractions of a Somatic Antigen of Trypanosoma cruzi

A delipidized somatic Trypanosoma cruzi antigen was mechanically fractionated in a Sephadex G-200 column, and the fractions were separated according to complement fixation (CF) (Fraction Ia) and indirect hemagglutination (IHA) activity (Fractions III, IV). The reaction of the active fractions agains...

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Bibliographic Details
Published in:The Journal of parasitology 1973-12, Vol.59 (6), p.1080-1084
Main Authors: Stella M. González Cappa, Kagan, Irving G.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
Antigens - analysis
Antigens - isolation & purification
Chagas Disease - blood
Chromatography, Gel
Complement Fixation Tests
DNA
DNA - analysis
Epimastigotes
Hemagglutination
Hemagglutination Tests
Humans
Immune Sera
Immunodiffusion
Nucleic acids
Parasitism
Parasitology
Proteins - analysis
Rabbits - immunology
Reactivity
RNA - analysis
Somatic antigens
Spectrophotometry
Trypanosoma cruzi - immunology
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Summary:A delipidized somatic Trypanosoma cruzi antigen was mechanically fractionated in a Sephadex G-200 column, and the fractions were separated according to complement fixation (CF) (Fraction Ia) and indirect hemagglutination (IHA) activity (Fractions III, IV). The reaction of the active fractions against chronic infected human sera was quite similar to the reaction of the unfractionated antigen. The CF active fraction was the only one with measurable amounts of DNA and was associated with the largest molecules. No precipitation activity was detected in this fraction. Precipitinogens had a wide size variation; they were present in the IHA active fractions as well as in other fractions. The two last fractions (VI and VII) did not show any antigenic activity.
ISSN:0022-3395
1937-2345
DOI:10.2307/3278646