Volatile profiles of toxigenic and non-toxigenic Aspergillus flavus using SPME for solid phase extraction

Toxigenic and atoxigenic strains of Aspergillus flavus were grown on potato dextrose agar (PDA) and wetted (23% moisture) sterile, cracked corn for 14 and 21 days, respectively. Volatile compounds produced by A. flavus, as well as those present in the PDA controls and sterile cracked maize, were col...

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Bibliographic Details
Published in:Annals of Agricultural and Environmental Medicine 2010, Vol.17 (2), p.301-308
Main Authors: De Lucca, Anthony J, Boué, Stephen M, Carter-Wientjes, Carol H, Bland, John M, Bhatnagar, Deepak, Cleveland, Thomas E
Format: Article
Language:English
Subjects:
Aspergillus flavus
Aspergillus flavus - chemistry
Corn
Dextrose
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
Mass spectroscopy
Potatoes
Solid phase methods
Solid Phase Microextraction - methods
Volatile Organic Compounds - analysis
Volatiles
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Summary:Toxigenic and atoxigenic strains of Aspergillus flavus were grown on potato dextrose agar (PDA) and wetted (23% moisture) sterile, cracked corn for 14 and 21 days, respectively. Volatile compounds produced by A. flavus, as well as those present in the PDA controls and sterile cracked maize, were collected using solid-phase micro-extraction (SPME) and identified by gas chromatography/mass spectrometry. Results show that growth substrate had a major impact on the number and type of volatiles detected. Growth on sterile cracked maize produced many more volatiles than did potato dextrose agar. There were also differences observed in the type of volatiles produced between toxigenic and non-toxigenic isolates, as well as between isolates of the same toxigenic grouping.
ISSN:1232-1966
1898-2263