PRELIMINARY STUDIES ON THE HISTOLOGICAL DEMONSTRATION OF DESOXYRIBONUCLEASE II BY ADAPTATION OF THE GOMORI ACID PHOSPHATASE METHOD

An attempt has been made to adapt the acid phosphatase method of Gomori to the demonstration of desoxyribonuclease II activity. The procedure as used is subject to the disadvantages of the original method. The enzymatic activity may be demonstrable only when desoxyribonuclease and nucleotidase activ...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1958-07, Vol.6 (4), p.255-259
Main Authors: ARONSON, J., HEMPELMANN, L. H., OKADA, S.
Format: Article
Language:English
Subjects:
Acid Phosphatase
Deoxyribonucleases
DNA - analysis
Endodeoxyribonucleases
Old Medline
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Summary:An attempt has been made to adapt the acid phosphatase method of Gomori to the demonstration of desoxyribonuclease II activity. The procedure as used is subject to the disadvantages of the original method. The enzymatic activity may be demonstrable only when desoxyribonuclease and nucleotidase activity occur in the same types of cells. Although the staining occurred mainly within discrete cells, the intracellular localization apparent under these conditions of study may well be artifact. In the spleen, the stainning occurs mainly in the red pulp and in a ring of cells, possibly macrophages, surrounding the lymphatic nodules. Hepatic and Kupffer cells stain as do cells in the tubules of the kidney cortex and in the intestinal mucosa.
ISSN:0022-1554
1551-5044
DOI:10.1177/6.4.255