Anti-oxidative effects of d-allose, a rare sugar, on ischemia-reperfusion damage following focal cerebral ischemia in rat

• Published in: Neuroscience letters 2011-01, Vol.487 (1), p.103-106
• Main Authors: Nakamura, Takehiro, Tanaka, Shinji, Hirooka, Kazuyuki, Toyoshima, Tetsuhiko, Kawai, Nobuyuki, Tamiya, Takashi, Shiraga, Fumio, Tokuda, Masaaki, Keep, Richard F., Itano, Toshifumi, Miyamoto, Osamu
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - therapeutic use; Behavior; Behavior, Animal; Biological and medical sciences; Brain Infarction - drug therapy; Brain Infarction - etiology; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - metabolism; Disease Models, Animal; Fundamental and applied biological sciences. Psychology; Glucose - therapeutic use; Infarction, Middle Cerebral Artery - drug therapy; Infarction, Middle Cerebral Artery - pathology; Infarction, Middle Cerebral Artery - physiopathology; Ischemia; Male; Medical sciences; Motor Activity - drug effects; Neurologic Examination; Neurology; Oxidation; Oxidative Stress - drug effects; Rare sugar; Rat; Rats; Rats, Sprague-Dawley; Reperfusion; Reperfusion Injury - drug therapy; Reperfusion Injury - pathology; Reperfusion Injury - physiopathology; Time Factors; Vascular diseases and vascular malformations of the nervous system; Vertebrates: nervous system and sense organs
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2010.10.004

▶ Brain damage after transient ischemia was decreased by d-allose administration. ▶ Oxidative stress markers were also reduced by d-allose. ▶ d-allose has anti-oxidative effects and induces neuroprotection in cerbral ischemia. The present study investigates the anti-oxidative effects of d-allose on ischemic damage. Rats were subjected to transient middle cerebral artery occlusion (MCAO) for 1 h under pentobarbital anesthesia. d-allose was intravenously infused during occlusion and a further 1 h after reperfusion (400 mg/kg). The effects of d-allose on focal cerebral ischemia were examined by measuring brain damage (infarction and atrophy volume) and behavioral deficits 7 days after MCAO. In another set of rats, apurnic/apyrimidic abasic sites (AP-sites) and 8-hydroxy-2′-deoxyguanosine (8-OHdG), oxidative stress markers, were investigated 24 h after MCAO to examine the anti-oxidative effects of d-allose. Brain damage and behavioral deficits were significantly decreased by d-allose administration compared to vehicle. The number of AP-sites and 8-OHdG levels were also reduced by d-allose. Thus, the present study suggests that d-allose has anti-oxidative effects and induces neuroprotection in focal cerebral ischemia.