The Structure of the Abortive Diphosphopyridine Nucleotide-Pyruvate-Lactate Dehydrogenase Ternary Complex as Determined by Proton Magnetic Resonance Analysis

The ternary complex, consisting of DPN+, pyruvate, and chicken heart lactate dehydrogenase, can be dissociated in base to yield a stable cyclic binary adduct. This DPNpyruvate adduct, which is a dihydropyridine derivative, is identical in structure with one of the two diastereomers produced chemical...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1974-01, Vol.249 (2), p.652-655
Main Authors: Arnold, Lyle J., Kaplan, Nathan O.
Format: Article
Language:English
Subjects:
Animals
Chemical Phenomena
Chemistry
Chickens
Drug Stability
Hydrogen-Ion Concentration
L-Lactate Dehydrogenase - antagonists & inhibitors
L-Lactate Dehydrogenase - metabolism
Magnetic Resonance Spectroscopy
Models, Chemical
Myocardium - enzymology
NAD - metabolism
Protons
Pyruvates - metabolism
Pyruvates - pharmacology
Spectrophotometry, Ultraviolet
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The ternary complex, consisting of DPN+, pyruvate, and chicken heart lactate dehydrogenase, can be dissociated in base to yield a stable cyclic binary adduct. This DPNpyruvate adduct, which is a dihydropyridine derivative, is identical in structure with one of the two diastereomers produced chemically by the addition of pyruvate to DPN+ in basic solution. A structural analysis of this enzyme-generated adduct by proton magnetic resonance has led to the following conclusions concerning the 322-nm-absorbing ternary complex of DPN+, enolpyruvate, and the lactate dehydrogenases: (a) a covalent bond forms between DPN+ and enolpyruvate; (b) the substitution of pyruvate on DPN+ occurs at the nicotinamide C4 position of DPN+; (c) the substitution takes place on the A side of the nicotinamide ring; and (d) this pyridine C4-substituted adduct of DPN+ is capable of dissociating intact from lactate dehydrogenase.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)43077-9