Enhanced production of validamycin A by H₂O₂-induced reactive oxygen species in fermentation of Streptomyces hygroscopicus 5008

A novel fermentation strategy to enhance antibiotics production was demonstrated by inducing reactive oxygen species (ROS), and validamycin A (VAL-A) production by Streptomyces hygroscopicus 5008 in agro-industrial residues containing medium was taken as an example. By optimizing H₂O₂ amount and add...

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Bibliographic Details
Published in:Bioresource technology 2011, Vol.102 (2), p.1783-1787
Main Authors: Wei, Zhen-Hua, Bai, Linquan, Deng, Zixin, Zhong, Jian-Jiang
Format: Article
Language:English
Subjects:
Fermentation - drug effects
Gene Expression Regulation, Bacterial - drug effects
Genes, Bacterial - genetics
Glucosephosphate Dehydrogenase - metabolism
Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism
Glycosyltransferases - genetics
Glycosyltransferases - metabolism
Hydrogen Peroxide - pharmacology
Inositol - analogs & derivatives
Inositol - biosynthesis
Intracellular Space - drug effects
Intracellular Space - metabolism
Onium Compounds - pharmacology
Streptomyces - drug effects
Streptomyces - enzymology
Streptomyces - genetics
Streptomyces - growth & development
Time Factors
Transcription, Genetic - drug effects
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Summary:A novel fermentation strategy to enhance antibiotics production was demonstrated by inducing reactive oxygen species (ROS), and validamycin A (VAL-A) production by Streptomyces hygroscopicus 5008 in agro-industrial residues containing medium was taken as an example. By optimizing H₂O₂ amount and addition time, the intracellular ROS level was increased, and VAL-A production titer was enhanced by 40% on day 4 when 25μM H₂O₂ was added at 8thh of fermentation. Addition of diphenyleneiodonium chloride (ROS inhibitor) reduced the H₂O₂ induction effect. The transcription level of eight VAL-A structure genes was enhanced by ROS, and activities of glucose-6-phosphate dehydrogenase and ValG enzyme were increased while glyceraldehyde 3-phosphate dehydrogenase activity was inhibited. This work demonstrated that ROS induction was a useful strategy for VAL-A fermentation, and the information on gene transcription and enzyme activities may be helpful to further understanding the mechanism of ROS effect on the antibiotic biosynthesis.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2010.08.114