Droplet microfluidics with magnetic beads: a new tool to investigate drug–protein interactions

In this study, we give the proof of concept for a method to determine binding constants of compounds in solution. By implementing a technique based on magnetic beads with a microfluidic device for segmented flow generation, we demonstrate, for individual droplets, fast, robust and complete separatio...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2011, Vol.399 (1), p.347-352
Main Authors: Lombardi, Dario, Dittrich, Petra S.
Format: Article
Language:English
Subjects:
Affinity labeling
Analysis
Analytical Chemistry
Anticoagulants - chemistry
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Design and construction
Dimethylpolysiloxane
Drops
Exact sciences and technology
Food Science
Humans
Iron oxides
Kinetics
Laboratory Medicine
Magnetic materials
Magnetic properties
Magnetics
Methods
Microelectromechanical systems
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidics
Monitoring/Environmental Analysis
Nanoparticles
Original Paper
Physiological aspects
Properties
Protein Binding
Separation (Technology)
Serum albumin
Serum Albumin - chemistry
Warfarin
Warfarin - chemistry
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Summary:In this study, we give the proof of concept for a method to determine binding constants of compounds in solution. By implementing a technique based on magnetic beads with a microfluidic device for segmented flow generation, we demonstrate, for individual droplets, fast, robust and complete separation of the magnetic beads. The beads are used as a carrier for one binding partner and hence, any bound molecule is separated likewise, while the segmentation into small microdroplets ensures fast mixing, and opens future prospects for droplet-wise analysis of drug candidate libraries. We employ the method for characterization of drug–protein binding, here warfarin to human serum albumin. The approach lays the basis for a microfluidic droplet-based screening device aimed at investigating the interactions of drugs with specific targets including enzymes and cells. Furthermore, the continuous method could be employed for various applications, such as binding assays, kinetic studies, and single cell analysis, in which rapid removal of a reactive component is required.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-010-4302-7