Single-cell manipulation on microfluidic chip by dielectrophoretic actuation and impedance detection

This paper presents the design, fabrication, and characterization of a microfluidic biochip with integrated actuation electrodes used to manipulate a cell and a microbead by dielectrophoresis and sensing electrodes to detect the trapping by using the impedance detection method. Combining deflective...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2010-09, Vol.150 (1), p.167-173
Main Authors: Park, Hyunjin, Kim, Dongil, Yun, Kwang-Seok
Format: Article
Language:English
Subjects:
Actuation
Detection
Dielectrophoresis
Electrodes
Impedance
Microfluidic device
Microfluidics
Microorganisms
Single-cell
Threshold voltage
Trapping
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Summary:This paper presents the design, fabrication, and characterization of a microfluidic biochip with integrated actuation electrodes used to manipulate a cell and a microbead by dielectrophoresis and sensing electrodes to detect the trapping by using the impedance detection method. Combining deflective dielectrophoretic barriers with controlled pressure-driven liquid flows allows the accurate control of a cell/microbead in suspensions. The threshold voltage for microbead trapping was experimentally verified at various flow rates. The impedance change caused by the blockage of the electrical conducting path between sensing electrodes with the trapping of an MCF7 cell and a polystyrene microbead was measured. The impedance before the trapping of an MCF7 cell was 10.9 MΩ at 1 kHz and increased to 12 MΩ when the cell was placed between sensing electrodes.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2010.07.020