Granulocyte Function in the Chediak-Higashi Syndrome of Mice

Granulocyte function was evaluated using peritoneal exudate granulocytes from a strain of beige mice with the Chediak-Higashi syndrome (CHS). Defective granulocyte Chemotaxis of CHS cells (43% normal) was documented. There was no abnormality in the ability of CHS serum to function as a chemotactic s...

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Bibliographic Details
Published in:Blood 1974-02, Vol.43 (2), p.201-206
Main Authors: Gallin, John I., Bujak, Joseph S., Patten, Ethel, Wolff, Sheldon M.
Format: Article
Language:English
Subjects:
Animals
Ascitic Fluid - cytology
Blood Bactericidal Activity
Carbon Radioisotopes
Caseins
Chediak-Higashi Syndrome - immunology
Chemotaxis
Chromium Radioisotopes
Escherichia coli - immunology
Female
Filtration
Leukocytes - immunology
Lysostaphin
Male
Mice
Mice, Inbred C57BL
Phagocytosis
Staphylococcus - immunology
Streptococcus - immunology
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Summary:Granulocyte function was evaluated using peritoneal exudate granulocytes from a strain of beige mice with the Chediak-Higashi syndrome (CHS). Defective granulocyte Chemotaxis of CHS cells (43% normal) was documented. There was no abnormality in the ability of CHS serum to function as a chemotactic stimulus. Phagocytosis of 14C-labeled Staphylococcus aureus by CHS granulocytes was normal. However, there was reduced bactericidal activity of 5. aureus and group D streptococcus by CHS cells through 90 min of incubation. The bactericidal defect was most pronounced at early time periods and was related to impaired intracellular killing. These defects of CHS mice granulocytes were analogous to those reported in man and it is concluded that the CHS mouse is a most convenient and representative model of the CHS of man.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V43.2.201.201