Measurement of Precipitin Reactions in the Millimicrogram Protein-Nitrogen Range

Exploitation of newer instrumentation and a dye-binding method for protein measurement made possible reduction in volume down to 1/1000 that commonly used, with no greater error. The procedure was tested at two levels, 1- and 10-μl volumes, with human gamma globulin and rabbit antiserum. Of the dyes...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1958-12, Vol.128 (3339), p.1625-1626
Main Authors: Glick, David, Good, Robert A., Greenberg, Leonard J., Eddy, Jennifer J., Day, Noorbibi K.
Format: Article
Language:English
Subjects:
Blood Proteins - analysis
Collagens
Coloring Agents
Dyes
Globulins
Humans
Lesions
Mating behavior
Nitrogen
Old Medline
Precipitates
Precipitins
Proteins
Sexual Behavior
Sodium
Solvents
Swelling
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Summary:Exploitation of newer instrumentation and a dye-binding method for protein measurement made possible reduction in volume down to 1/1000 that commonly used, with no greater error. The procedure was tested at two levels, 1- and 10-μl volumes, with human gamma globulin and rabbit antiserum. Of the dyes tested, bromsulfalein proved best for the protein estimation.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.128.3339.1625