Chromosome Assignment of the Human Gene for Galactose-1-phosphate Uridyltransferase

Somatic cell hybrids formed between galactose-negative mutant Chinese hamster cells and human lymphocytes were selected in galactose medium and analyzed for the concurrent presence of specific human chromosome(s) and human galactose-1-phosphate uridyltransferase. In one of the hybrid clones only a s...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-02, Vol.71 (2), p.404-407
Main Authors: Sun, N. C., Chang, C. C., Chu, E. H. Y.
Format: Article
Language:English
Subjects:
Animals
Biological Sciences: Genetics
Burkitt Lymphoma
Cell fusion
Cell Line
Cell lines
Chromosome Mapping
Chromosomes
Cricetinae
Galactose
Genes
Hamsters
Hexosephosphates
Human chromosomes
Humans
Hybrid Cells
Hybridity
Karyotyping
Lymphocytes
Nucleotidyltransferases - biosynthesis
Somatic cells
Uracil Nucleotides
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Summary:Somatic cell hybrids formed between galactose-negative mutant Chinese hamster cells and human lymphocytes were selected in galactose medium and analyzed for the concurrent presence of specific human chromosome(s) and human galactose-1-phosphate uridyltransferase. In one of the hybrid clones only a single human chromosome, A2, was consistently present when the hybrid cells were maintained in the selective medium, suggesting that the location of the structural gene for galactose-1-phosphate uridyltransferase is on this chromosome. Among subclones derived from this cell hybrid in the absence of selection, a complete concordance was demonstrated between the ability or inability to utilize galactose and the concomitant presence or absence of the human transferase and the A2 chromosome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.2.404