Studies on Delayed (Cellular) Hypersensitivity in Mice Infected with Trichinella spiralis. IX. Delayed Dermal Sensitivity in Artificially Sensitized Donors

A series of preliminary experiments was completed to check skin-testing techniques with both our crude saline extract antigen and Melcher's antigen. Some of the sensitized mice that had been injected into the abdominal skin with test doses of 20 and 100 µg of trichinella protein developed what...

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Bibliographic Details
Published in:The Journal of parasitology 1974-02, Vol.60 (1), p.93-98
Main Authors: Larsh, John E., Weatherly, Norman F.
Format: Article
Language:English
Subjects:
Animals
Antigens
Antigens - administration & dosage
Antigens - isolation & purification
Delayed hypersensitivity
Freund's Adjuvant - administration & dosage
Hypersensitivity, Delayed - immunology
Immunity, Cellular
Immunization
Infections
Injections
Injections, Intradermal
Larva - immunology
Larvae
Parasitism
Parasitology
Sensitization
Skin - immunology
Skin Tests
Spleen cells
Swelling
Trichinella - immunology
Trichinellosis - immunology
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Summary:A series of preliminary experiments was completed to check skin-testing techniques with both our crude saline extract antigen and Melcher's antigen. Some of the sensitized mice that had been injected into the abdominal skin with test doses of 20 and 100 µg of trichinella protein developed what appeared to be positive DH reactions, but reactions in some controls, due presumably to nonspecific irritation produced by particulate matter, made differentiation uncertain. In the 2 final experiments, Melcher's antigen and the footpad swelling test were used and the results with peak reactions at 24 hr were unequivocal. It required 7 days after completion of sensitization to obtain consistently strong reactions, and 2 sensitizations produced much more swelling than one. Proof for a characteristic DH response was obtained in tissue sections that showed a predominant infiltration of mononuclear cells.
ISSN:0022-3395
1937-2345
DOI:10.2307/3278685