Evidence for the Participation of Cytochrome b5 in Plasmalogen Biosynthesis

Using a specific antibody to rat liver cytochrome b5, ethanolamine plasmalogen (1-O-alk-1′-enyl-2-acyl-sn-glycerophosphorylethanolamine) synthesis by pork spleen microsomes is shown to require cytochrome b5. Either NADH or NADPH could serve as a source of reducing equivalents. These results provide...

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Bibliographic Details
Published in:The Journal of biological chemistry 1974-04, Vol.249 (8), p.2661-2662
Main Authors: Paltauf, Fritz, Prough, Russell A., Masters, Bettie Sue Siler, Johnston, John M.
Format: Article
Language:English
Subjects:
Animals
Cross Reactions
Cytochrome Reductases - antagonists & inhibitors
Cytochromes - metabolism
Electron Transport - drug effects
Immunoglobulins
Male
Microsomes - drug effects
Microsomes - enzymology
Microsomes - metabolism
Mixed Function Oxygenases - antagonists & inhibitors
NAD - metabolism
NADP - metabolism
Oxidation-Reduction
Plasmalogens - biosynthesis
Rabbits - immunology
Rats
Spleen - drug effects
Spleen - enzymology
Spleen - metabolism
Swine
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Summary:Using a specific antibody to rat liver cytochrome b5, ethanolamine plasmalogen (1-O-alk-1′-enyl-2-acyl-sn-glycerophosphorylethanolamine) synthesis by pork spleen microsomes is shown to require cytochrome b5. Either NADH or NADPH could serve as a source of reducing equivalents. These results provide direct evidence for the involvement of cytochrome b5 in plasmalogen biosynthesis from the corresponding 1-O-alkyl ether and suggest a pathway analogous to the fatty acid desaturase system.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)42782-8