Evaluation of Thiosulfate-Citrate-Bile Salts-Sucrose Agar, a Selective Medium for the Isolation of Vibrio cholerae and Other Pathogenic Vibrios

Three brands of thiosulfate-citrate-bile salts-sucrose (TCBS) agar, a selective medium for the isolation of Vibrio cholerae and other pathogenic vibrios, were compared. Although the three products supported the growth of V. cholerae and Vibrio parahemolyticus equally well, there were lot-to-lot and...

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Bibliographic Details
Published in:The Journal of infectious diseases 1974-05, Vol.129 (5), p.497-500
Main Authors: McCormack, William M., DeWitt, Wallis E., Bailey, Penrhyn E., Morris, George K., Soeharjono, Padkodiwirju, Gangarosa, Eugene J.
Format: Article
Language:English
Subjects:
Agar
Bacteriology
Bile Acids and Salts
Blood
Cholera
Cholera - microbiology
Citrates
Culture Media
Disease outbreaks
Epidemiology
Feces - microbiology
Gelatins
Humans
Major Articles
Methods
Southeast Asian culture
Specimens
Sucrose
Thiosulfates
Vibrio
Vibrio - isolation & purification
Vibrio cholerae
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Summary:Three brands of thiosulfate-citrate-bile salts-sucrose (TCBS) agar, a selective medium for the isolation of Vibrio cholerae and other pathogenic vibrios, were compared. Although the three products supported the growth of V. cholerae and Vibrio parahemolyticus equally well, there were lot-to-lot and brand-to-brand variations in the ability of these media to inhibit organisms present in the bowel flora. In general, TCBS agar produced by Eiken Chemical Company was more effective in this regard than TCBS agar produced by Baltimore Biological Laboratories or Difco Laboratories.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/129.5.497