Study of CoQ10-Enzymes in Gingiva from Patients with Periodontal Disease and Evidence for a Deficiency of Coenzyme Q10

The specific activities of both the succinate dehydrogenase-coenzyme Q10reductase and the DPNH-cytochrome c reductase [NADH:(acceptor)oxidoreductase, EC 1.6.99.3] were determined in mitochondria from 40 diseased gingival biopsies from patients with periodontal disease and from 24 control biopsies fr...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-04, Vol.71 (4), p.1456-1460
Main Authors: Nakamura, Ryo, Littarru, Gian Paolo, Folkers, Karl, Wilkinson, Edward G.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Biochemistry
Biological Sciences: Medical Sciences
Biopsies
Chromium
Coenzymes
Cytochrome c Group
Cytochrome Reductases - metabolism
Enzyme activity
Enzymes
Female
Gingiva
Gingiva - enzymology
Gingival Diseases - enzymology
Humans
Male
Middle Aged
Mitochondria
Mitochondria - enzymology
NADH, NADPH Oxidoreductases - metabolism
Periodontal diseases
Periodontics
Succinate Dehydrogenase - metabolism
Ubiquinone - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The specific activities of both the succinate dehydrogenase-coenzyme Q10reductase and the DPNH-cytochrome c reductase [NADH:(acceptor)oxidoreductase, EC 1.6.99.3] were determined in mitochondria from 40 diseased gingival biopsies from patients with periodontal disease and from 24 control biopsies from non-diseased areas (clinically evaluated) of gingival tissues from the same mouths of the patients from whom the diseased gingival tissues were taken. The control tissue was taken during normal surgical procedures, such as for gingival recontouring and tuberosity removal. The diseased gingival biopsies showed a mean specific activity for the succinate dehydrogenase-coenzyme Q10reductase which was higher (P < 0.02) than that of the control biopsies, and which increased (P < 0.01) when the assays utilized exogenous coenzyme Q3, and corresponded to an average deficiency of coenzyme Q10-enzyme activity of 35%. About 60% of the 40 diseased gingival tissues showed a deficiency of coenzyme Q10at its site in this succinate-coenzyme Q10enzyme. Of the 24 control tissues, 20% showed deficiencies of coenzyme Q10. As a group, the control tissues showed no deficiency of coenzyme Q10. No deficiency of coenzyme Q10at its site in DPNH-cytochrome c reductase was observed for either the control or diseased gingival tissues, as groups or individually.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.71.4.1456